Evidence map›Paper›PMID 29044103›Full record

ArticleNature communications2017

OCT4 impedes cell fate redirection by the melanocyte lineage master regulator MITF in mouse ESCs.

Danna Sheinboim, Itay Maza, Iris Dror, Shivang Parikh, Vladislav Krupalnik, Rachel E Bell, Asaf Zviran, Yusuke Suita, Ofir Hakim, Yael Mandel-Gutfreund and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 7 institutions in 3 countries.

Danna SheinboimDepartment of Human Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel.
Itay MazaDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, 7610001, Israel.
Iris DrorDepartment of Biology, Technion-Israel Institute of Technology, Haifa, 32000, Israel.
Shivang ParikhDepartment of Human Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel.
Vladislav KrupalnikDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, 7610001, Israel.
Rachel E BellDepartment of Human Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel.
Asaf ZviranDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, 7610001, Israel.
Yusuke SuitaCutaneous Biology Research Center, Department of Dermatology and MGH Cancer Center, Massachusetts General Hospital, Boston, MA, 02114, USA.
Ofir HakimThe Mina and Everard Goodman Faculty of Life Sciences Bar-Ilan University, Ramat Gan, 5290002, Israel.
Yael Mandel-GutfreundDepartment of Biology, Technion-Israel Institute of Technology, Haifa, 32000, Israel.
Mehdi KhaledInstitut Gustave Roussy, INSERM 1186, Villejuif, 94805, France.
Jacob H HannaDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, 7610001, Israel. jacob.hanna@weizmann.ac.il.ORCID http://orcid.org/0000-0003-2042-9974
Carmit LevyDepartment of Human Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel. carmitlevy@post.tau.ac.il.
Tel Aviv University · ILWeizmann Institute of Science · ILTechnion – Israel Institute of Technology · ILBar-Ilan University · ILInserm · FRMassachusetts General Hospital · USUniversity of California, Los Angeles · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ectopic expression of lineage master regulators induces transdifferentiation. Whether cell fate transitions can be induced during various developmental stages has not been systemically examined. Here we discover that amongst different developmental stages, mouse embryonic stem cells (mESCs) are resistant to cell fate conversion induced by the melanocyte lineage master regulator MITF. By generating a transgenic system we exhibit that in mESCs, the pluripotency master regulator Oct4, counteracts pro-differentiation induced by Mitf by physical interference with MITF transcriptional activity. We further demonstrate that mESCs must be released from Oct4-maintained pluripotency prior to ectopically induced differentiation. Moreover, Oct4 induction in various differentiated cells represses their lineage identity in vivo. Alongside, chromatin architecture combined with ChIP-seq analysis suggest that Oct4 competes with various lineage master regulators for binding promoters and enhancers. Our analysis reveals pluripotency and transdifferentiation regulatory principles and could open new opportunities in the field of regenerative medicine.

Indexed as

AnimalsCell DifferentiationCell Line, TumorCells, CulturedCell TransdifferentiationEmbryo, MammalianFibroblastsGene Expression ProfilingGene Expression Regulation, DevelopmentalHumansMiceMice, TransgenicMicrophthalmia-Associated Transcription FactorMouse Embryonic Stem CellsOctamer Transcription Factor-3Microphthalmia-Associated Transcription FactorOctamer Transcription Factor-3Pou5f1 protein, mouse

Identifiers

PMID29044103
PMCPMC5647326
OpenAlexW2761518775

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.