ArticleMicrobiology (Reading, England)2017
N-acetylglucosamine affects Cryptococcus neoformans cell-wall composition and melanin architecture.
Article in Microbiology (Reading, England), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Transcription factor RonA-driven GlcNAc catabolism is essential for growth, cell wall integrity, and pathogenicity inMicrobiology spectrum · 2025Article
- Dietary L-3,4-dihydroxyphenylalanine (L-DOPA) augments cuticular melanization in Anopheles mosquitos reducing their lifespan and malaria burden.Nature communications · 2025Article
- Fungal melanin suppresses airway epithelial chemokine secretion through blockade of calcium fluxing.Nature communications · 2024Article
- Rise and fall of Caspofungin: the current status of Caspofungin as a treatment forFuture microbiology · 2024Review
- The Transcription Factor CcRlm1 Regulates Cell Wall Maintenance and Poplar Defense Response by Directly TargetingApplied and environmental microbiology · 2023Article
- Melanization ofJournal of fungi (Basel, Switzerland) · 2022Article
- Wor1-regulated ferroxidases contribute to pigment formation in opaque cells of Candida albicans.FEBS open bio · 2021Article
- Pyomelanin Synthesis inFrontiers in microbiology · 2021Article
- Unconventional Constituents and Shared Molecular Architecture of the Melanized Cell Wall ofJournal of fungi (Basel, Switzerland) · 2020Article
- Solid-state NMR spectroscopy identifies three classes of lipids inThe Journal of biological chemistry · 2020Article
- N-Acetylglucosamine (GlcNAc) Sensing, Utilization, and Functions inJournal of fungi (Basel, Switzerland) · 2020Review
- Melanin deposition in twoThe Journal of biological chemistry · 2020Article
- The Transcriptomic and Phenotypic Response of the Melanized YeastFrontiers in microbiology · 2020Article
- Review
- Article
- The structural unit of melanin in the cell wall of the fungal pathogenThe Journal of biological chemistry · 2019Article
- Molecular Mechanisms of Chitosan Interactions with Fungi and Plants.International journal of molecular sciences · 2019Review
- The Fungal Cell Wall:Frontiers in microbiology · 2019Review
- The melanization road more traveled by: Precursor substrate effects on melanin synthesis in cell-free and fungal cell systems.The Journal of biological chemistry · 2018Article
- Cell-wall dyes interfere with Cryptococcus neoformans melanin deposition.Microbiology (Reading, England) · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Cryptococcus neoformans is an environmental fungus that belongs to the phylum Basidiomycetes and is a major pathogen in immunocompromised patients. The ability of C. neoformans to produce melanin pigments represents its second most important virulence factor, after the presence of a polysaccharide capsule. Both the capsule and melanin are closely associated with the fungal cell wall, a complex structure that is essential for maintaining cell morphology and viability under conditions of stress. The amino sugar N-acetylglucosamine (GlcNAc) is a key constituent of the cell-wall chitin and is used for both N-linked glycosylation and GPI anchor synthesis. Recent studies have suggested additional roles for GlcNAc as an activator and mediator of cellular signalling in fungal and plant cells. Furthermore, chitin and chitosan polysaccharides interact with melanin pigments in the cell wall and have been found to be essential for melanization. Despite the importance of melanin, its molecular structure remains unresolved; however, we previously obtained critical insights using advanced nuclear magnetic resonance (NMR) and imaging techniques. In this study, we investigated the effect of GlcNAc supplementation on cryptococcal cell-wall composition and melanization. C. neoformans was able to metabolize GlcNAc as a sole source of carbon and nitrogen, indicating a capacity to use a component of a highly abundant polymer in the biospherenutritionally. C. neoformans cells grown with GlcNAc manifested changes in the chitosan cell-wall content, cell-wall thickness and capsule size. Supplementing cultures with isotopically
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.