Evidence map›Paper›PMID 29043661›Full record

ArticleApplied biochemistry and biotechnology2018

Development of Adenosine Deaminase-Specific IgY Antibodies: Diagnostic and Inhibitory Application.

Agnieszka Łupicka-Słowik, Mateusz Psurski, Renata Grzywa, Kamila Bobrek, Patrycja Smok, Maciej Walczak, Andrzej Gaweł, Tadeusz Stefaniak, Józef Oleksyszyn, Marcin Sieńczyk

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied biochemistry and biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

  1. IgY Antibodies from Birds: A Review on Affinity and Avidity.Animals : an open access journal from MDPI · 2023
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Agnieszka Łupicka-SłowikFaculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland.
Mateusz PsurskiLaboratory of Experimental Anticancer Therapy, Department of Experimental Oncology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114, Wrocław, Poland.
Renata GrzywaFaculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland.
Kamila BobrekFaculty of Veterinary Medicine, Department of Epizootiology and Clinic of Bird and Exotic Animals, Wroclaw University of Environmental and Life Sciences, Pl. Grunwaldzki 45, 50-366, Wrocław, Poland.
Patrycja SmokFaculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland.
Maciej WalczakFaculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland.
Andrzej GawełFaculty of Veterinary Medicine, Department of Epizootiology and Clinic of Bird and Exotic Animals, Wroclaw University of Environmental and Life Sciences, Pl. Grunwaldzki 45, 50-366, Wrocław, Poland.
Tadeusz StefaniakFaculty of Veterinary Medicine, Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375, Wrocław, Poland.
Józef OleksyszynFaculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland.
Marcin SieńczykFaculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland. marcin.sienczyk@pwr.edu.pl.
Wrocław University of Science and Technology · PLWroclaw University of Environmental and Life Sciences · PLAGH University of Krakow · PLPolish Academy of Sciences · PL

Funding

Narodowe Centrum Badań i Rozwoju LIDER/08/90/l-1/09/NCBiR/2010Politechnika Wrocławska Statute Funds 0401/0250/16
6 · The paper itself

Abstract

Adenosine deaminase (ADA) is currently used as a diagnostic marker for tuberculous pleuritis. Although ADA has been suggested as a potential marker for several types of cancer, the importance of each of ADA isoforms as well as their levels and enzymatic activities in tumors need to be further investigated. Herein we developed avian immunoglobulin Y highly specific to human ADA via hens immunization with calf adenosine deaminase. The obtained antibodies were used for the development of a sensitive double-egg yolk immunoglobulin (IgY) sandwich ELISA assay with an ADA detection limit of 0.5 ng/ml and a linearity range of up to 10 ng/ml. Specific, affinity-purified IgYs were able to recognize human recombinant ADA and ADA present in human cancer cell lines. In addition, antigen-specific IgY antibodies were able to inhibit catalytic activity of calf ADA with an IC

Indexed as

Antibodies, NeoplasmAntibody SpecificityAvian ProteinsImmunoglobulinsAdenosine DeaminaseAnimalsCattleCell Line, TumorChickensHumansNeoplasm ProteinsNeoplasmsAdenosine DeaminaseAntibodies, NeoplasmAvian ProteinsIgYImmunoglobulinsNeoplasm ProteinsAdenosine deaminase (ADA)Affinity purificationAnti-adenosine deaminase antibodyEgg yolk immunoglobulin (IgY)ELISAEnzyme inhibition

Identifiers

PMID29043661
PMCPMC5889419
OpenAlexW2765533364

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.