ArticleFrontiers in physiology2017
Influence of Standard Laboratory Procedures on Measures of Erythrocyte Damage.
Article in Frontiers in physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Efficacy and Safety of Vortexing as a Practical Solution for Resolving Platelet Clumping in hematology laboratories.International journal of laboratory hematology · 2026Article
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- In Vitro Evaluation of the Effect of Stenting on Hematological, Hemorheological and Hemodynamic Parameters, in Various Stent Configurations and Flow Conditions.Cardiovascular engineering and technology · 2025Article
- Article
- Experimental Investigation of the Applicability of the Stress-Based and Strain-Based Hemolysis Models for Short-Term Stress Peaks Typical for Rotary Blood Pumps.Artificial organs · 2025Article
- Mean Platelet Volume in Blood vs. Platelet-Rich Plasma: Demographic Analysis and Intrasubject Variability in PRP Therapy.The archives of bone and joint surgery · 2025Article
- How to alleviate cardiac injury from electric shocks at the cellular level.Frontiers in cardiovascular medicine · 2022Article
- New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density.Bioactive materials · 2021Article
- Donor Age and Red Cell Age Contribute to the Variance in Lorrca Indices in Healthy Donors for Next Generation Ektacytometry: A Pilot Study.Frontiers in physiology · 2021Article
- Glutaraldehyde - A Subtle Tool in the Investigation of Healthy and Pathologic Red Blood Cells.Frontiers in physiology · 2019Article
- Centrifugation-induced release of ATP from red blood cells.PloS one · 2018Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to characterize the mechanical properties of erythrocytes is important in clinical and research contexts: to diagnose and monitor hematologic disorders, as well as to optimize the design of cardiovascular implants and blood circulating devices with respect to blood damage. However, investigation of red blood cell (RBC) properties generally involves preparatory and processing steps. Even though these impose mechanical stresses on cells, little is known about their impact on the final measurement results. In this study, we investigated the effect of centrifuging, vortexing, pipetting, and high pressures on several markers of mechanical blood damage and RBC membrane properties. Using human venous blood, we analyzed erythrocyte damage by measuring free hemoglobin, phosphatidylserine exposure by flow cytometry, RBC deformability by ektacytometry and the parameters of a complete blood count. We observed increased levels of free hemoglobin for all tested procedures. The release of hemoglobin into plasma depended significantly on the level of stress. Elevated pressures and centrifuging also altered mean cell volume (MCV) and mean corpuscular hemoglobin (MCH), suggesting changes in erythrocyte population, and membrane properties. Our results show that the effects of blood handling can significantly influence erythrocyte damage metrics. Careful quantification of this influence as well as other unwanted secondary effects should thus be included in experimental protocols and accounted for in clinical laboratories.
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