ArticleScientific reports2017
A single point mutation in class III ribonucleotide reductase promoter renders Pseudomonas aeruginosa PAO1 inefficient for anaerobic growth and infection.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Ribonucleotide reductase repression: a mutational study of NrdR-binding motifs.Journal of bacteriology · 2026Article
- Interplay ofVirulence · 2025Article
- FleQ-Dependent regulation of the ribonucleotide reductase repressor NrdR in Pseudomonas aeruginosa during biofilm growth and infection.Scientific reports · 2025Article
- Optimized alveolar epithelial cell model for chroniciScience · 2025Article
- Who arrived first? Priority effects on Candida albicans and Pseudomonas aeruginosa dual biofilms.Communications biology · 2025Article
- Detection of Known and Novel Small Proteins inAnalytical chemistry · 2023Article
- Characterization and Functional Analysis of a New Calcium/Calmodulin-Dependent Protein Kinase (CaMK1) in the Citrus Pathogenic FungusJournal of fungi (Basel, Switzerland) · 2022Article
- A new BiofilmChip device for testing biofilm formation and antibiotic susceptibility.NPJ biofilms and microbiomes · 2021Article
- The Small RNA ErsA Impacts the Anaerobic Metabolism ofFrontiers in microbiology · 2021Article
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- Monitoring Gene Expression during aMicroorganisms · 2020Article
- Optimal environmental and culture conditions allow the in vitro coexistence of Pseudomonas aeruginosa and Staphylococcus aureus in stable biofilms.Scientific reports · 2019Article
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- Aerobic Vitamin BFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas aeruginosa strain PAO1 has become the reference strain in many laboratories. One enzyme that is essential for its cell division is the ribonucleotide reductase (RNR) enzyme that supplies the deoxynucleotides required for DNA synthesis and repair. P. aeruginosa is one of the few microorganisms that encodes three different RNR classes (Ia, II and III) in its genome, enabling it to grow and adapt to diverse environmental conditions, including during infection. In this work, we demonstrate that a lack of RNR activity induces cell elongation in P. aeruginosa PAO1. Moreover, RNR gene expression during anaerobiosis differs among P. aeruginosa strains, with class III highly expressed in P. aeruginosa clinical isolates relative to the laboratory P. aeruginosa PAO1 strain. A single point mutation was identified in the P. aeruginosa PAO1 strain class III RNR promoter region that disrupts its anaerobic transcription by the Dnr regulator. An engineered strain that induces the class III RNR expression allows P. aeruginosa PAO1 anaerobic growth and increases its virulence to resemble that of clinical strains. Our results demonstrate that P. aeruginosa PAO1 is adapted to laboratory conditions and is not the best reference strain for anaerobic or infection studies.
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