ArticleBiochemistry2018
Comparative Membrane Proteomics Reveals a Nonannotated E. coli Heat Shock Protein.
Article in Biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Cellular function of the GndA microprotein during heat shock.bioRxiv : the preprint server for biology · 2025Article
- Eukaryotic Microproteins.Annual review of biochemistry · 2025Review
- Plastid Molecular Chaperone HSP90C Interacts with the SecA1 Subunit of Sec Translocase for Thylakoid Protein Transport.Plants (Basel, Switzerland) · 2024Article
- Biophysical characterization of high-confidence, small human proteins.bioRxiv : the preprint server for biology · 2024Article
- Microproteins-Discovery, structure, and function.Proteomics · 2023Review
- Unannotated microprotein EMBOW regulates the interactome and chromatin and mitotic functions of WDR5.Cell reports · 2023Article
- Chemical labeling and proteomics for characterization of unannotated small and alternative open reading frame-encoded polypeptides.Biochemical Society transactions · 2023Review
- Rescue ofeLife · 2023Article
- Engineered multiple translation initiation sites: a novel tool to enhance protein production in Bacillus licheniformis and other industrially relevant bacteria.Nucleic acids research · 2022Article
- Hidden in plain sight: challenges in proteomics detection of small ORF-encoded polypeptides.microLife · 2022Article
- Towards the characterization of the hidden world of small proteins in Staphylococcus aureus, a proteogenomics approach.PLoS genetics · 2021Article
- Identification of Translation Start Sites in Bacterial Genomes.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Comparative Proteomic Profiling of Unannotated Microproteins and Alternative Proteins in Human Cell Lines.Journal of proteome research · 2020Article
- Non-AUG start codons: Expanding and regulating the small and alternative ORFeome.Experimental cell research · 2020Review
- Review
- Alternative ORFs and small ORFs: shedding light on the dark proteome.Nucleic acids research · 2020Review
- Proteomic Detection and Validation of Translated Small Open Reading Frames.Current protocols in chemical biology · 2019Article
- Retapamulin-Assisted Ribosome Profiling Reveals the Alternative Bacterial Proteome.Molecular cell · 2019Article
- Small open reading frames and cellular stress responses.Molecular omics · 2019Review
- Genes within Genes in Bacterial Genomes.Microbiology spectrum · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Recent advances in proteomics and genomics have enabled discovery of thousands of previously nonannotated small open reading frames (smORFs) in genomes across evolutionary space. Furthermore, quantitative mass spectrometry has recently been applied to analysis of regulated smORF expression. However, bottom-up proteomics has remained relatively insensitive to membrane proteins, suggesting they may have been underdetected in previous studies. In this report, we add biochemical membrane protein enrichment to our previously developed label-free quantitative proteomics protocol, revealing a never-before-identified heat shock protein in Escherichia coli K12. This putative smORF-encoded heat shock protein, GndA, is likely to be ∼36-55 amino acids in length and contains a predicted transmembrane helix. We validate heat shock-regulated expression of the gndA smORF and demonstrate that a GndA-GFP fusion protein cofractionates with the cell membrane. Quantitative membrane proteomics therefore has the ability to reveal nonannotated small proteins that may play roles in bacterial stress responses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.