ArticleScientific reports2017
Simultaneous visualisation of calcified bone microstructure and intracortical vasculature using synchrotron X-ray phase contrast-enhanced tomography.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 38 citations in OpenAlex.
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- Evidence for peri-lacunar remodeling and altered osteocyte lacuno-canalicular network in mouse models of myeloma-induced bone disease.JBMR plus · 2024Article
- Osteocyte mitochondria regulate angiogenesis of transcortical vessels.Nature communications · 2024Article
- Multiscale morphological analysis of bone microarchitecture around Mg-10Gd implants.Bioactive materials · 2023Article
- Computed Tomography as a Characterization Tool for Engineered Scaffolds with Biomedical Applications.Materials (Basel, Switzerland) · 2021Review
- A robust, semi-automated approach for counting cementum increments imaged with synchrotron X-ray computed tomography.PloS one · 2021Article
- X-ray computed tomography in life sciences.BMC biology · 2020Review
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- Risperidone-Loaded PLGA-Lipid Particles with Improved Release Kinetics: Manufacturing and Detailed Characterization by Electron Microscopy and Nano-CT.Pharmaceutics · 2019Article
- In vivo delivery of VEGF RNA and protein to increase osteogenesis and intraosseous angiogenesis.Scientific reports · 2019Article
- Regulation of the Bone Vascular Network is Sexually Dimorphic.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Article
- 3-dimensional visualization of implant-tissue interface with the polyethylene glycol associated solvent system tissue clearing method.Cell proliferation · 2019Article
- Visualising the Cardiovascular System of Embryos of Biomedical Model Organisms with High Resolution Episcopic Microscopy (HREM).Journal of cardiovascular development and disease · 2018Review
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Authors and funding
6 authors at 3 institutions in 4 countries.
Funding
Abstract
3D imaging of the bone vasculature is of key importance in the understanding of skeletal disease. As blood vessels in bone are deeply encased in the calcified matrix, imaging techniques that are applicable to soft tissues are generally difficult or impossible to apply to the skeleton. While canals in cortical bone can readily be identified and characterised in X-ray computed tomographic data in 3D, the soft tissue comprising blood vessels that are putatively contained within the canal structures does not provide sufficient image contrast necessary for image segmentation. Here, we report an approach that allows for rapid, simultaneous visualisation of calcified bone tissue and the vasculature within the calcified bone matrix. Using synchrotron X-ray phase contrast-enhanced tomography we show exemplar data with intracortical capillaries uncovered at sub-micrometre level without the need for any staining or contrast agent. Using the tibiofibular junction of 15 week-old C57BL/6 mice post mortem, we show the bone cortical porosity simultaneously along with the soft tissue comprising the vasculature. Validation with histology confirms that we can resolve individual capillaries. This imaging approach could be easily applied to other skeletal sites and transgenic models, and could improve our understanding of the role the vasculature plays in bone disease.
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Registered trials
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