ArticleScientific reports2017
Structural variability and complexity of the giant Pithovirus sibericum particle revealed by high-voltage electron cryo-tomography and energy-filtered electron cryo-microscopy.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Template Learning: Deep learning with domain randomization for particle picking in cryo-electron tomography.Nature communications · 2025Article
- Refining the taxonomy of pithovirus-related giant DNA viruses within the order Pimascovirales.Archives of virology · 2025Article
- Review
- The future of Asian biophysics built through Korea-Japan collaboration.Biophysics and physicobiology · 2025Article
- Analysis of the Genomic Features and Evolutionary History of Pithovirus-Like Isolates Reveals Two Major Divergent Groups of Viruses.Journal of virology · 2023Article
- The Astounding World of Glycans from Giant Viruses.Chemical reviews · 2022Review
- Quantitative Cryo-Electron Tomography.Frontiers in molecular biosciences · 2022Review
- The Secret Life of Giant Viruses in the California Current.mSystems · 2021Article
- Near-Field Vortex Beams Diffraction on Surface Micro-Defects and Diffractive Axicons for Polarization State Recognition.Sensors (Basel, Switzerland) · 2021Article
- Review
- Advances in Cryo-Correlative Light and Electron Microscopy: Applications for Studying Molecular and Cellular Events.The protein journal · 2019Review
- Review
- Correlative light electron microscopy of giant viruses with the SECOM system.New microbes and new infections · 2018Article
- Morphologic and Genomic Analyses of New Isolates Reveal a Second Lineage of Cedratviruses.Journal of virology · 2018Article
- Cedratvirus getuliensis replication cycle: an in-depth morphological analysis.Scientific reports · 2018Article
- Review
- A Large Open Pangenome and a Small Core Genome for Giant Pandoraviruses.Frontiers in microbiology · 2018Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Pithoviridae giant virus family exhibits the largest viral particle known so far, a prolate spheroid up to 2.5 μm in length and 0.9 μm in diameter. These particles show significant variations in size. Little is known about the structure of the intact virion due to technical limitations with conventional electron cryo-microscopy (cryo-EM) when imaging thick specimens. Here we present the intact structure of the giant Pithovirus sibericum particle at near native conditions using high-voltage electron cryo-tomography (cryo-ET) and energy-filtered cryo-EM. We detected a previously undescribed low-density outer layer covering the tegument and a periodical structuring of the fibres in the striated apical cork. Energy-filtered Zernike phase-contrast cryo-EM images show distinct substructures inside the particles, implicating an internal compartmentalisation. The density of the interior volume of Pithovirus particles is three quarters lower than that of the Mimivirus. However, it is remarkably high given that the 600 kbp Pithovirus genome is only half the size of the Mimivirus genome and is packaged in a volume up to 100 times larger. These observations suggest that the interior is densely packed with macromolecules in addition to the genomic nucleic acid.
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