Evidence map›Paper›PMID 29038534›Full record

ArticleScientific reports2017

Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice.

Mohamed Abdo Rizk, Shimaa Abd El-Salam El-Sayed, Mahmoud AbouLaila, Rasha Eltaysh, Naoaki Yokoyama, Ikuo Igarashi

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
3.5field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 34 citations in OpenAlex.

  1. Epidemiological and Molecular Characteristics ofTransboundary and emerging diseases · 2024
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  2. Pathogens and global health · 2023
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  3. Evaluation of the Inhibitory Effect ofJournal of tropical medicine · 2023
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  6. Evaluation of theJournal of advanced veterinary and animal research · 2022
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  7. Pomegranate (Journal of parasitology research · 2022
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  8. Effect of methanolic extract fromVeterinary world · 2022
    Article
  9. Discovering the Potent Inhibitors AgainstFrontiers in veterinary science · 2021
    Article
  10. Article
  11. Myrrh OilPathogens (Basel, Switzerland) · 2020
    Article
  12. Article
  13. Article
  14. Inhibitory effects of fluoroquinolone antibiotics onInfection and drug resistance · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Mohamed Abdo RizkNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, 080-8555, Japan.
Shimaa Abd El-Salam El-SayedNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, 080-8555, Japan.
Mahmoud AbouLailaNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, 080-8555, Japan.
Rasha EltayshNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, 080-8555, Japan.
Naoaki YokoyamaNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, 080-8555, Japan.
Ikuo IgarashiNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, 080-8555, Japan. igarcpmi@obihiro.ac.jp.
Obihiro University of Agriculture and Veterinary Medicine · JPMansoura University · EGUniversity of Sadat City · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we evaluated the validity of a fluorescence-based assay using SYBR Green I (SG I) stain for screening antibabesial compounds against B. microti in mice. Two different hematocrits (HCTs; 2.5% and 5%) were used. Correlating relative fluorescence units (RFUs) with parasitemia showed significant linear relationships with R

Indexed as

Drug Evaluation, PreclinicalAnemiaAnimalsAntiprotozoal AgentsBabesiaBenzothiazolesDiaminesDiminazeneDrug Therapy, CombinationFemaleFluorescenceHematocritHigh-Throughput Screening AssaysLeukocytesMice, Inbred BALB CNaphthyridinesAntiprotozoal AgentsBenzothiazolesDiaminesDiminazenediminazene aceturateNaphthyridinesNucleic AcidsOrganic ChemicalspyronaridineQuinolinesSYBR Green I

Identifiers

PMID29038534
PMCPMC5643553
OpenAlexW2763356662

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.