ArticleScientific reports2017
The Bacteroidales produce an N-acylated derivative of glycine with both cholesterol-solubilising and hemolytic activity.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 42 citations in OpenAlex.
- Endocannabinoids and related lipids in host metabolism-gut microbiome signalling.Nature reviews. Endocrinology · 2026Review
- Ornithine lipids and other acyloxyacyl amino lipids: the coming-of-age story of a group of non-canonical membrane lipids.Applied microbiology and biotechnology · 2026Review
- Dynamic Changes in Intestinal Microorganisms and Hematological Indices in Giraffes of Different Ages, and the Effect of Diarrhea on Intestinal Microbiota.Animals : an open access journal from MDPI · 2024Article
- What defines a healthy gut microbiome?Gut · 2024Review
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- Synthesis ofMolecules (Basel, Switzerland) · 2024Article
- Aminolipids in bacterial membranes and the natural environment.The ISME journal · 2024Review
- Eucalyptus essential oil exerted a sedative-hypnotic effect by influencing brain neurotransmitters and gut microbes via the gut microbiota-brain axis.Frontiers in pharmacology · 2024Article
- Article
- Construction of a Bacterial Lipidomics Analytical Platform: Pilot Validation with Bovine Paratuberculosis Serum.Metabolites · 2023Article
- The roles of dietary lipids and lipidomics in gut-brain axis in type 2 diabetes mellitus.Journal of translational medicine · 2023Review
- Membrane lipids from gut microbiome-associated bacteria as structural and signalling molecules.Microbiology (Reading, England) · 2023Review
- Lipidomic Analysis Reveals Differences inMetabolites · 2023Article
- Gut commensals and their metabolites in health and disease.Frontiers in microbiology · 2023Review
- The regulatory effects of second-generation antipsychotics on lipid metabolism: Potential mechanisms mediated by the gut microbiota and therapeutic implications.Frontiers in pharmacology · 2023Review
- Analysis of the oral microbiome during hormonal cycle and its alterations in menopausal women: the "AMICA" project.Scientific reports · 2022Article
- Lipidomics Analysis of Outer Membrane Vesicles and Elucidation of the Inositol Phosphoceramide Biosynthetic Pathway in Bacteroides thetaiotaomicron.Microbiology spectrum · 2022Article
- Facile and Sustainable Synthesis of Commendamide and its Analogues.Frontiers in chemistry · 2022Article
- Glycine acylation and trafficking of a new class of bacterial lipoprotein by a composite secretion system.eLife · 2021Article
- Linking Gut Microbiome and Lipid Metabolism: Moving beyond Associations.Metabolites · 2021Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The contribution of the gut microbiota to the metabolism of cholesterol is not well understood. In this study, we identify 21 fosmid clones from a human gut microbiome metagenomic library that, when expressed in Escherichia coli, produce halos on LB agar supplemented with 0.01% (w/v) cholesterol (LBC agar). Analysis of 14 of these clones revealed that they all share a fragment of DNA with homology to the genome of Bacteroides vulgatus. The gene responsible for halo production on LBC agar, named choA, was identified as an N-acyltransferase known to produce an acylated glycine molecule called commendamide. In this study we show that commendamide is capable of producing a halo on LBC agar suggesting that this molecule is solubilizing the cholesterol micelles in LBC agar. We also show that commendamide is responsible for the previously described hemolytic activity associated with the choA orthologue in Bacteroides fragilis. A functional analysis of ChoA identified 2 amino acids that are important for commendamide biosynthesis and we present phylogenetic and functional data showing that orthologues of choA are found only in the order Bacteroidales. Therefore, the production of commendamide may be an adaptation to the environments colonized by the Bacteroidales, including the mammalian gut.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.