Evidence map›Paper›PMID 29035321›Full record

ArticleInternational journal of molecular sciences2017

A Novel Multi-Epitope Vaccine Based on Urate Transporter 1 Alleviates Streptozotocin-Induced Diabetes by Producing Anti-URAT1 Antibody and an Immunomodulatory Effect in C57BL/6J Mice.

Yanjie Ma, Huimin Cao, Zhixin Li, Jinzhi Fang, Xiaomin Wei, Peng Cheng, Rui Jiao, Xiaoran Liu, Ya Li, Yun Xing and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Yanjie MaSchool of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China. 14211030526@stu.cpu.edu.cn.
Huimin CaoSchool of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China. 1621030544@stu.cpu.edu.cn.
Zhixin LiSchool of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China. 14211030601@stu.cpu.edu.cn.
Jinzhi FangSchool of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China. 2020140639@stu.cpu.edu.cn.
Xiaomin WeiSchool of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China. 13211030585@stu.cpu.edu.cn.
Peng ChengSchool of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China. 15211030512@stu.cpu.edu.cn.
Rui JiaoSchool of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China. 15211030561@stu.cpu.edu.cn.
Xiaoran LiuSchool of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China. 14211030603@stu.cpu.edu.cn.
Ya LiSchool of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China. 1621030486@stu.cpu.edu.cn.
Yun XingSchool of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China. xingyun_503@163.com.
Jiali TangSchool of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China. 13645195250@163.com.
Liang JinSchool of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China. liangjin1975@cpu.edu.cn.
Taiming LiSchool of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China. taimingli@163.com.
China Pharmaceutical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hyperuricemia (HUA) is related to diabetes. Uric acid-induced inflammation and oxidative stress are risk factors for diabetes and its complications. Human urate transporter 1 (URAT1) regulates the renal tubular reabsorption of uric acid. IA-2(5)-P2-1, a potent immunogenic carrier designed by our laboratory, can induce high-titer specific antibodies when it carries a B cell epitope, such as B cell epitopes of DPP4 (Dipeptidyl peptidase-4), xanthine oxidase. In this report, we describe a novel multi-epitope vaccine composing a peptide of URAT1, an anti-diabetic B epitope of insulinoma antigen-2(IA-2) and a Th2 epitope (P2:IPALDSLTPANED) of P277 peptide in human heat shock protein 60 (HSP60). Immunization with the multi-epitope vaccine in streptozotocin-induced diabetes C57BL/6J mice successfully induced specific anti-URAT1 antibody, which inhibited URAT1 action and uric acid reabsorption, and increased pancreatic insulin level with a lower insulitis incidence. Vaccination with U-IA-2(5)-P2-1 (UIP-1) significantly reduced blood glucose and uric acid level, increased Th2 cytokines interleukin (IL)-10 and IL-4, and regulated immune reactions through a balanced Th1/Th2 ratio. These results demonstrate that the URAT1-based multi-epitope peptide vaccine may be a suitable therapeutic approach for diabetes and its complications.

Indexed as

ImmunomodulationAnimalsAntioxidantsAutoantibodiesAutoantigensCytokinesDiabetes Mellitus, ExperimentalDiabetes Mellitus, Type 1EpitopesGlucoseHyperglycemiaHyperuricemiaImmunoglobulin GIslets of LangerhansMalondialdehydeMiceAntioxidantsAutoantibodiesAutoantigensCytokinesEpitopesGlucoseImmunoglobulin GMalondialdehydeOrganic Anion TransportersSlc22a12 protein, mouseSuperoxide DismutaseUric AcidVaccinesantibodydiabeteshyperuricemiaurate transporter 1uric acid

Identifiers

PMID29035321
PMCPMC5666819
OpenAlexW2767012374

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.