Evidence map›Paper›PMID 29032467›Full record

ArticleCurrent microbiology2018

A Helicobacter pylori Vacuolating Cytotoxin A: Mouse DHFR Fusion Protein Triggers Dye Release from Liposomes.

Aung Khine Linn, Nitchakan Samainukul, Somsri Sakdee, Chanan Angsuthanasombat, Gerd Katzenmeier

Abstract read
PubMed Publisher
In one paragraph

Article in Current microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.2field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Aung Khine LinnBacterial Protein Toxin Research Cluster, Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25 Phutthamonthon 4 Road, Salaya, Nakhon Pathom, 73170, Thailand.
Nitchakan SamainukulBacterial Protein Toxin Research Cluster, Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25 Phutthamonthon 4 Road, Salaya, Nakhon Pathom, 73170, Thailand.
Somsri SakdeeBacterial Protein Toxin Research Cluster, Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25 Phutthamonthon 4 Road, Salaya, Nakhon Pathom, 73170, Thailand.
Chanan AngsuthanasombatBacterial Protein Toxin Research Cluster, Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25 Phutthamonthon 4 Road, Salaya, Nakhon Pathom, 73170, Thailand.
Gerd KatzenmeierBacterial Protein Toxin Research Cluster, Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25 Phutthamonthon 4 Road, Salaya, Nakhon Pathom, 73170, Thailand. katzenmeier.ger@mahidol.ac.th.
Mahidol University · TH

Funding

Thailand Research Fund RSA5580047
6 · The paper itself

Abstract

The membrane perturbing action of the VacA toxin from Helicobacter pylori is responsible for vacuole formation in intracellular compartments and the induction of apoptosis. The VacA toxin contains 2 major domains, p33 and p55, which are involved in receptor binding and membrane pore formation, respectively. Improved methodologies for VacA purification and assays are urgently needed for further detailed investigations on the mechanism of action of this significant virulence factor. We found that by fusing mouse DHFR with the N-terminus of the full-length (p88) VacA toxin, expression levels in recombinant E. coli were substantially increased when compared to the conventional (His)

Indexed as

AnimalsBacterial ProteinsColoring AgentsEscherichia coliGene ExpressionLiposomesMembranesMiceRecombinant Fusion ProteinsTetrahydrofolate DehydrogenaseBacterial ProteinsColoring AgentsLiposomesRecombinant Fusion ProteinsTetrahydrofolate DehydrogenaseVacA protein, Helicobacter pyloriDihydrofolate reductaseFusion proteinHelicobacter pyloriLiposomeVacuolating cytotoxin A (VacA)

Identifiers

PMID29032467
OpenAlexW2761433350

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.