Evidence map›Paper›PMID 29032234›Full record

ArticleMitochondrion2018

Saccharomyces cerevisiae Mhr1 can bind Xho I-induced mitochondrial DNA double-strand breaks in vivo.

Kanchanjunga Prasai, Lucy C Robinson, Kelly Tatchell, Lynn Harrison

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mitochondrion, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Kanchanjunga PrasaiDepartment of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA.
Lucy C RobinsonDepartment of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA.
Kelly TatchellDepartment of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA.
Lynn HarrisonDepartment of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA. Electronic address: lclary@lsuhsc.edu.
Louisiana State University Health Sciences Center Shreveport · US

Funding

Enhancing radiation and cisplatin HNSCC cell killing by inhibiting mitochondrialR21CA167796 · NCI · LOUISIANA STATE UNIV HSC SHREVEPORT · PI HARRISON, LYNN · 2012 to 2013
$332k
NCI NIH HHS R21 CA167796
6 · The paper itself

Abstract

Mitochondrial DNA (mtDNA) double-strand break (DSB) repair is essential for maintaining mtDNA integrity, but little is known about the proteins involved in mtDNA DSB repair. Here, we utilize Saccharomyces cerevisiae as a eukaryotic model to identify proteins involved in mtDNA DSB repair. We show that Mhr1, a protein known to possess homologous DNA pairing activity in vitro, binds to mtDNA DSBs in vivo, indicating its involvement in mtDNA DSB repair. Our data also indicate that Yku80, a protein previously implicated in mtDNA DSB repair, does not compete with Mhr1 for binding to mtDNA DSBs. In fact, C-terminally tagged Yku80 could not be detected in yeast mitochondrial extracts. Therefore, we conclude that Mhr1, but not Yku80, is a potential mtDNA DSB repair factor in yeast.

Indexed as

DNA Breaks, Double-StrandedDNA RepairDNA-Binding ProteinsDNA, FungalDNA, MitochondrialSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription FactorsDNA-Binding ProteinsDNA, FungalDNA, MitochondrialMHR1 protein, S cerevisiaeSaccharomyces cerevisiae ProteinsTranscription FactorsYKU80 protein, S cerevisiaeDouble strand break repairMhr1Mitochondrial DNAMitochondrial DNA immunoprecipitationYku80

Identifiers

PMID29032234
PMCPMC6159889
OpenAlexW2762006536

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.