ArticleMolecular microbiology2017
Spatial transcriptomes within the Pseudomonas aeruginosa biofilm architecture.
Article in Molecular microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 48 citations in OpenAlex.
- Characterizing Spatiotemporal Expansion of Pseudomonas aeruginosa Communities in Polymer and Mucin Gel Environments.Biotechnology and bioengineering · 2026Article
- Dispersal Mechanisms in Biofilm Control: Characteristics, Induction, Impacts, and Therapeutic Potential.Current microbiology · 2025Review
- FleQ finetunes the expression of a subset of BrlR-activated genes to enable antibiotic tolerance byJournal of bacteriology · 2025Article
- How Often Does Bacteremia Occur in Patients With Chronic Periprosthetic Joint Infection? A Prospective, Observational Study.Clinical orthopaedics and related research · 2025Observational
- Biofilms as more than the sum of their parts: lessons from developmental biology.Current opinion in microbiology · 2024Review
- Deciphering the influence of NaCl on social behaviour ofRoyal Society open science · 2024Article
- Review
- Membrane fluidity homeostasis is required for tobramycin-enhanced biofilm inMicrobiology spectrum · 2024Article
- Origins of symbiosis: shared mechanisms underlying microbial pathogenesis, commensalism and mutualism of plants and animals.FEMS microbiology reviews · 2023Review
- What's in a name? Characteristics of clinical biofilms.FEMS microbiology reviews · 2023Article
- Spatial transcriptome uncovers rich coordination of metabolism in E. coli K12 biofilm.Nature chemical biology · 2023Article
- MoaB1 Homologs Contribute to Biofilm Formation and Motility by Pseudomonas aeruginosa and Escherichia coli.Journal of bacteriology · 2023Article
- How to study biofilms: technological advancements in clinical biofilm research.Frontiers in cellular and infection microbiology · 2023Review
- The biofilm life cycle: expanding the conceptual model of biofilm formation.Nature reviews. Microbiology · 2022Review
- The Role of Staphylococcal Biofilm on the Surface of Implants in Orthopedic Infection.Microorganisms · 2022Review
- Nutrient Sensing and Biofilm Modulation: The Example of L-arginine inInternational journal of molecular sciences · 2022Review
- Controlling Biofilm Development Through Cyclic di-GMP Signaling.Advances in experimental medicine and biology · 2022Review
- Flagella, Chemotaxis and Surface Sensing.Advances in experimental medicine and biology · 2022Article
- SagS and its unorthodox contributions toBiofilm · 2021Review
- Advances and opportunities in image analysis of bacterial cells and communities.FEMS microbiology reviews · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors at 5 institutions in 2 countries.
Funding
Abstract
Bacterial cooperative associations and dynamics in biofilm microenvironments are of special interest in recent years. Knowledge of localized gene-expression and corresponding bacterial behaviors within the biofilm architecture at a global scale has been limited, due to a lack of robust technology to study limited number of cells in stratified layers of biofilms. With our recent pioneering developments in single bacterial cell transcriptomic analysis technology, we generated herein an unprecedented spatial transcriptome map of the mature in vitro Pseudomonas aeruginosa biofilm model, revealing contemporaneous yet altered bacterial behaviors at different layers within the biofilm architecture (i.e., surface, middle and interior of the biofilm). Many genes encoding unknown functions were highly expressed at the biofilm-solid interphase, exposing a critical gap in the knowledge of their activities that may be unique to this interior niche. Several genes of unknown functions are critical for biofilm formation. The in vivo importance of these unknown proteins was validated in invertebrate (fruit fly) and vertebrate (mouse) models. We envisage the future value of this report to the community, in aiding the further pathophysiological understanding of P. aeruginosa biofilms. Our approach will open doors to the study of bacterial functional genomics of different species in numerous settings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.