Evidence map›Paper›PMID 29030743›Full record

ArticleDigestive diseases and sciences2017

miR-182 and miR-135b Mediate the Tumorigenesis and Invasiveness of Colorectal Cancer Cells via Targeting ST6GALNAC2 and PI3K/AKT Pathway.

Li Jia, Shihua Luo, Xiang Ren, Yang Li, Jialei Hu, Bing Liu, Lifen Zhao, Yujia Shan, Huimin Zhou

Abstract read
PubMed Publisher
In one paragraph

Article in Digestive diseases and sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
3.5field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 74 citations in OpenAlex.

  1. Review
  2. Article
  3. Lgr6Genes & diseases · 2026
    Review
  4. Article
  5. Review
  6. Review
  7. Review
  8. Review
  9. Review
  10. Review
  11. Review
  12. Article
  13. Article
  14. Article
  15. Review
  16. Review
  17. Review
  18. Review
  19. The Role of miRNA-182 and FOXO3 Expression in Breast Cancer.Asian Pacific journal of cancer prevention : APJCP · 2022
    Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Li JiaCollege of Laboratory Medicine, Dalian Medical University, Dalian, 116044, Liaoning Province, China. jiali0386@sina.com.ORCID 0000-0003-3152-3253
Shihua LuoCollege of Laboratory Medicine, Dalian Medical University, Dalian, 116044, Liaoning Province, China.
Xiang RenCollege of Stomatology, Dalian Medical University, Dalian, 116044, Liaoning Province, China.
Yang LiCollege of Laboratory Medicine, Dalian Medical University, Dalian, 116044, Liaoning Province, China.
Jialei HuCollege of Laboratory Medicine, Dalian Medical University, Dalian, 116044, Liaoning Province, China.
Bing LiuCollege of Laboratory Medicine, Dalian Medical University, Dalian, 116044, Liaoning Province, China.
Lifen ZhaoCollege of Laboratory Medicine, Dalian Medical University, Dalian, 116044, Liaoning Province, China.
Yujia ShanCollege of Laboratory Medicine, Dalian Medical University, Dalian, 116044, Liaoning Province, China.
Huimin ZhouCollege of Laboratory Medicine, Dalian Medical University, Dalian, 116044, Liaoning Province, China.
Dalian Medical University · CN

Funding

National Natural Science Foundation of China 81472014
6 · The paper itself

Abstract

backgroundMetastasis is a leading cause of cancer-related death including colorectal cancer (CRC). MicroRNAs are known to regulate cancer pathways and to be expressed aberrantly in cancer. Aberrant sialylation is closely associated with malignant phenotype of tumor cells, including invasiveness and metastasis.

aimThis study aimed to investigate the association of miR-182 and miR-135b with proliferation and invasion by targeting sialyltransferase ST6GALNAC2 in CRC cells and explore the potential molecular mechanism.

methodsWe measured the levels of miR-182, miR-135b, and ST6GALNAC2 in a series of CRC cell lines and tissues using real-time PCR. Bioinformatics analysis and luciferase reporter assay were performed to test the direct binding of miR-182 and miR-135b to the target gene ST6GALNAC2. We also analyzed the possible role of miR-182/-135b on colony formation, wound healing, invasion, and tube formation.

resultsThe expression of miR-182 and miR-135b was higher in tumor tissues compared to adjacent noncancerous tissues of CRC patients, as well as up-regulated in SW620 cells than in SW480 cells with different metastatic potential. By applying bioinformatics analysis and luciferase reporter assay, we identified ST6GALNAC2 as the direct target of miR-182/-135b. Furthermore, miR-182/-135b inhibited significantly ST6GALNAC2 expression, and consistently, ST6GALNAC2 mediated migration, adhesion, invasion, proliferation, and tumor angiogenesis in CRC cell lines. Additionally, PI3K/AKT signaling pathway was regulated by miR-182/135b, which was partially blocked by altered level of ST6GALNAC2 in CRC.

conclusionsThe miR-182/-135b/ST6GALNAC2/PI3K/AKT axis may serve as a predictive biomarker and a potential therapeutic target in CRC treatment.

Indexed as

AnimalsCarcinogenesisCell LineColorectal NeoplasmsMaleMice, NudeMicroRNAsNeoplasm InvasivenessPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSialyltransferasesMicroRNAsMIRN135 microRNA, humanMirn182 microRNA, humanPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSialyltransferasesST6GALNAC2 protein, humanColorectal cancerMetastasismicroRNAsPI3K/AKTST6GALNAC2

Identifiers

PMID29030743
OpenAlexW2763981440

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.