ArticleThe EMBO journal1988
Uptake of GABA by rat brain synaptic vesicles isolated by a new procedure.
Article in The EMBO journal, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 173 citations in OpenAlex.
- Article
- Cell-Type-Specific Neuroproteomics of Synapses.Biomolecules · 2023Review
- Cross-linking mass spectrometry uncovers protein interactions and functional assemblies in synaptic vesicle membranes.Nature communications · 2021Article
- Perturbation of the Vacuolar ATPase: A NOVEL CONSEQUENCE OF INOSITOL DEPLETION.The Journal of biological chemistry · 2015Article
- Synaptic vesicles contain small ribonucleic acids (sRNAs) including transfer RNA fragments (trfRNA) and microRNAs (miRNA).Scientific reports · 2015Article
- Sorting of the vesicular GABA transporter to functional vesicle pools by an atypical dileucine-like motif.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Small-scale isolation of synaptic vesicles from mammalian brain.Nature protocols · 2013Article
- Post-translational modifications and lipid binding profile of insect cell-expressed full-length mammalian synaptotagmin 1.Biochemistry · 2011Article
- Determining the number of specific proteins in cellular compartments by quantitative microscopy.Nature protocols · 2011Article
- Pressure-driven laminar flow switching for rapid exchange of solution environment around surface adhered biological particles.Lab on a chip · 2010Article
- Vesicular inhibitory amino acid transporter is a Cl-/gamma-aminobutyrate Co-transporter.The Journal of biological chemistry · 2009Article
- Alzheimer's disease protein Abeta1-42 does not disrupt isolated synaptic vesicles.Biochimica et biophysica acta · 2008Article
- Sizing subcellular organelles and nanoparticles confined within aqueous droplets.Analytical chemistry · 2008Article
- Deconvolving single-molecule intensity distributions for quantitative microscopy measurements.Biophysical journal · 2007Article
- Presynaptic regulation of quantal size by the vesicular glutamate transporter VGLUT1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Identification of the differentiation-associated Na+/PI transporter as a novel vesicular glutamate transporter expressed in a distinct set of glutamatergic synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Ca2+ sensitivity of synaptic vesicle dopamine, gamma-aminobutyric acid, and glutamate transport systems.Neurochemical research · 2001Article
- Immunoisolation of GABA-specific synaptic vesicles defines a functionally distinct subset of synaptic vesicles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Aczonin, a 550-kD putative scaffolding protein of presynaptic active zones, shares homology regions with Rim and Bassoon and binds profilin.The Journal of cell biology · 1999Article
- Paralemmin, a prenyl-palmitoyl-anchored phosphoprotein abundant in neurons and implicated in plasma membrane dynamics and cell process formation.The Journal of cell biology · 1998Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Uptake of GABA was demonstrated in rat brain synaptic vesicles which were prepared by a new and efficient procedure. The uptake activity co-purified with the synaptic vesicles during the isolation procedure. The purity of the vesicle fraction was rigorously examined by analysis of marker enzymes and marker proteins and also by immunogold electron microscopy using antibodies against p38 (synaptophysin). Contamination by other cellular components was negligible, indicating that GABA uptake by the synaptic vesicle fraction is specific for synaptic vesicles and not due to the presence of other structure possessing GABA uptake or binding activities. GABA uptake was ATP dependent and similar to the uptake of glutamate, which was assayed for a comparison. Both uptake activities were independent of sodium. They were inhibited by the uncoupler carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, indicating that the energy for the uptake is provided by an electrochemical proton gradient. This gradient is generated by a proton ATPase of the vacuolar type as suggested by the effects of various ATPase inhibitors on neurotransmitter uptake and proton pumping. Competition experiments revealed that the transporters for GABA and glutamate are selective for the respective neurotransmitters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.