ArticleJournal of virological methods2018
Single reaction, real time RT-PCR detection of all known avian and human metapneumoviruses.
Article in Journal of virological methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Visual and Equipment-Minimized Detection of Avian Metapneumovirus Subtype C by RT-RPA-CRISPR/Cas12a Coupled with Lateral Flow Dipstick.Animals : an open access journal from MDPI · 2026Article
- Avian metapneumovirus: A five-plex digital droplet RT-PCR method for identification of subgroups A, B, C, and D.Frontiers in veterinary science · 2022Article
- Acetic acid alleviates the inflammatory response and liver injury in septic mice by increasing the expression of TRIM40.Experimental and therapeutic medicine · 2019Article
- Development of a Sensitive Real-Time Fast-qPCR Based on SYBRVeterinary sciences · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current molecular methods for the detection of avian and human metapneumovirus (AMPV, HMPV) are specifically targeted towards each virus species or individual subgroups of these. Here a broad range SYBR Green I real time RT-PCR was developed which amplified a highly conserved fragment of sequence in the N open reading frame. This method was sufficiently efficient and specific in detecting all MPVs. Its validation according to the NF U47-600 norm for the four AMPV subgroups estimated low limits of detection between 1000 and 10copies/μL, similar with detection levels described previously for real time RT-PCRs targeting specific subgroups. RNA viruses present a challenge for the design of durable molecular diagnostic test due to the rate of change in their genome sequences which can vary substantially in different areas and over time. The fact that the regions of sequence for primer hybridization in the described method have remained sufficiently conserved since the AMPV and HMPV diverged, should give the best chance of continued detection of current subgroups and of potential unknown or future emerging MPV strains.
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