ArticlePloS one2017
Diversity and microevolution of CRISPR loci in Helicobacter cinaedi.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- SpacerPlacer: ancestral reconstruction of CRISPR arrays reveals the evolutionary dynamics of spacer deletions.Nucleic acids research · 2024Article
- Analysis of CRISPR/Cas Genetic Structure, Spacer Content and Molecular Epidemiology in BrazilianPathogens (Basel, Switzerland) · 2023Article
- Article
- A genomic sequence of the type II-A clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system inJournal of oral microbiology · 2022Article
- Association of CRISPR-Cas System with the Antibiotic Resistance and Virulence Genes in Nosocomial Isolates ofInfection and drug resistance · 2022Article
- Long-run bacteria-phage coexistence dynamics under natural habitat conditions in an environmental biotechnology system.The ISME journal · 2021Article
- Occurrence and Diversity of CRISPR Loci inFrontiers in microbiology · 2020Article
- The ecology and evolution of microbial CRISPR-Cas adaptive immune systems.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Article
- Multi-step genomic dissection of a suspected intra-hospital Helicobacter cinaedi outbreak.Microbial genomics · 2018Article
- Article
- Study the Features of 57 Confirmed CRISPR Loci in 38 Strains ofFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Helicobacter cinaedi is associated with nosocomial infections. The CRISPR-Cas system provides adaptive immunity against foreign genetic elements. We investigated the CRISPR-Cas system in H. cinaedi to assess the potential of the CRISPR-based microevolution of H. cinaedi strains. A genotyping method based on CRISPR spacer organization was carried out using 42 H. cinaedi strains. The results of sequence analysis showed that the H. cinaedi strains used in this study had two CRISPR loci (CRISPR1 and CRISPR2). The lengths of the consensus direct repeat sequences in CRISPR1 and CRISPR2 were both 36 bp-long, and 224 spacers were found in the 42 H. cinaedi strains. Analysis of the organization and sequence similarity of the spacers of the H. cinaedi strains showed that CRISPR arrays could be divided into 7 different genotypes. Each genotype had a different ancestral spacer, and spacer acquisition/deletion events occurred while isolates were spreading. Spacer polymorphisms of conserved arrays across the strains were instrumental for differentiating closely-related strains collected from the same hospital. MLST had little variability, while the CRISPR sequences showed remarkable diversity. Our data revealed the structural features of H. cinaedi CRISPR loci for the first time. CRISPR sequences constitute a valuable basis for genotyping, provide insights into the divergence and relatedness between closely-related strains, and reflect the microevolutionary process of H. cinaedi.
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