ArticleeLife2017
RNA polymerase II stalling at pre-mRNA splice sites is enforced by ubiquitination of the catalytic subunit.
Article in eLife, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Article
- Redundant pathways for removal of defective RNA polymerase II complexes at a promoter-proximal pause checkpoint.Molecular cell · 2024Article
- Truncating the spliceosomal 'rope protein' Prp45 results in Htz1 dependent phenotypes.RNA biology · 2024Article
- Regulation of Pre-mRNA Splicing: Indispensable Role of Post-Translational Modifications of Splicing Factors.Life (Basel, Switzerland) · 2023Review
- Editorial: RNA machines.Frontiers in genetics · 2023Article
- RNA polymerase II speed: a key player in controlling and adapting transcriptome composition.The EMBO journal · 2021Review
- Co-transcriptional splicing regulates 3' end cleavage during mammalian erythropoiesis.Molecular cell · 2021Article
- Article
- A negative feedback mechanism links UBC gene expression to ubiquitin levels by affecting RNA splicing rather than transcription.Scientific reports · 2019Article
- Nascent RNA and the Coordination of Splicing with Transcription.Cold Spring Harbor perspectives in biology · 2019Review
- Analysis of RNA polymerase II ubiquitylation and proteasomal degradation.Methods (San Diego, Calif.) · 2019Article
- Long-read sequencing of nascent RNA reveals coupling among RNA processing events.Genome research · 2018Article
- When SUMO met splicing.RNA biology · 2018Review
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
Numerous links exist between co-transcriptional RNA processing and the transcribing RNAPII. In particular, pre-mRNA splicing was reported to be associated with slowed RNAPII elongation. Here, we identify a site of ubiquitination (K1246) in the catalytic subunit of RNAPII close to the DNA entry path. Ubiquitination was increased in the absence of the Bre5-Ubp3 ubiquitin protease complex. Bre5 binds RNA in vivo, with a preference for exon 2 regions of intron-containing pre-mRNAs and poly(A) proximal sites. Ubiquitinated RNAPII showed similar enrichment. The absence of Bre5 led to impaired splicing and defects in RNAPII elongation in vivo on a splicing reporter construct. Strains expressing RNAPII with a K
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Registered trials
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