ArticleJournal of bone and mineral metabolism2018
Impact of flow shear stress on morphology of osteoblast-like IDG-SW3 cells.
Article in Journal of bone and mineral metabolism, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- A quantitative meta-analysis comparing cell models in perfused organ on a chip with static cell cultures.Scientific reports · 2023Pooled it
- Bionic Nanostructures Create Mechanical Signals to Mediate the Composite Structural Bone Regeneration Through Multi-System Regulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Spatial regulation of hydrogel polymerization reaction using ultrasound-driven streaming vortex.Ultrasonics sonochemistry · 2024Article
- β-glycerophosphate, not low magnitude fluid shear stress, increases osteocytogenesis in the osteoblast-to-osteocyte cell line IDG-SW3.Connective tissue research · 2024Article
- Effects of compound stimulation of fluid shear stress plus ultrasound on stem cell proliferation and osteogenesis.Regenerative biomaterials · 2021Article
- The Measurement and Analysis of Impedance Response of HeLa Cells to Distinct Chemotherapy Drugs.Micromachines · 2021Article
- IDG-SW3 Osteocyte Differentiation and Bone Extracellular Matrix Deposition Are Enhanced in a 3D Matrix Metalloproteinase-Sensitive Hydrogel.ACS applied bio materials · 2020Article
- Article
- [Optimization of the theoretical model for growth rate of mesenchymal stem cells on three-dimensional scaffold under fluid shear stress].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2019Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
This study constructed an in situ cell culture, real-time observation system based originally on a microfluidic channel, and reported the morphological changes of late osteoblast-like IDG-SW3 cells in response to flow shear stress (FSS). The effects of high (1.2 Pa) and low (0.3 Pa) magnitudes of unidirectional FSS and three concentrations of extracellular Type I collagen (0.1, 0.5, and 1 mg/mL) coating on cell morphology were investigated. IDG-SW3 cells were cultured in polydimethylsiloxane microfluidic channels. Cell images were recorded real-time under microscope at intervals of 1 min. Cell morphology was characterized by five parameters: cellular area, cell elongation index, cellular alignment, cellular process length, and number of cellular process per cell. Immunofluorescence assay was used to detect stress fiber distribution and vinculin expression. The results showed that 1.2 Pa, but not 0.3 Pa of FSS induced a significant morphological change in late osteoblast-like IDG-SW3 cells, which may be caused by the alteration of cellular adhesion with matrix in response to FSS. Moreover, the amount of collagen matrix, alignment of fiber stress and expression of vinculin were closely correlated with the morphological changes of IDG-SW3 cells. This study suggests that osteoblasts are very responsive to the magnitudes of FSS, and extracellular collagen matrix and focal adhesion are directly involved in the morphological changes adaptive to FSS.
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Registered trials
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