ArticlePlant methods2017
An optimized method for high quality DNA extraction from microalga
Article in Plant methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Integrated Screening and Antifungal Potential of Microalgae Extracts Against Botrytis californica.Current microbiology · 2026Article
- Recovery and Survival of Prototheca Microalgae During Wastewater Treatment Process.Microbial biotechnology · 2026Article
- Two high-quality genomes of Prototheca bovis strain SH08 and Prototheca ciferrii strain SH13.Scientific data · 2025Article
- Endogenized polinton-like viruses in the dinoflagellateThe Journal of general virology · 2025Article
- Mechanism of salt tolerance ability of novel Desertifilum salkalinema SSAU 7 for sustainable development.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Molecular and Phenotypic Characterization ofAnimals : an open access journal from MDPI · 2025Article
- Complete mitochondrial genomes of the Prototheca genus: comparative genomics and evolutionary insights.BMC genomics · 2025Article
- Breaking the paradigm-Applied and environmental microbiology · 2025Article
- Reduced plastid genomes of colorless facultative pathogens Prototheca (Chlorophyta) are retained for membrane transport genes.BMC biology · 2024Article
- Chromosome-scale assembly of the streamlined picoeukaryoteMicrobial genomics · 2024Article
- Simple and Effective Squash-PCR for Rapid Genotyping of Industrial Microalgae.Life (Basel, Switzerland) · 2024Article
- A domesticated photoautotrophic microbial community as a biofilm model system for analyzing the influence of plastic surfaces on invertebrate grazers in limnic environments.Frontiers in microbiology · 2023Article
- Occurrence ofApplied and environmental microbiology · 2022Article
- Effects of Extracellular Self- and Nonself-DNA on the Freshwater MicroalgaPlants (Basel, Switzerland) · 2022Article
- Genome Sequences of Two Strains ofFrontiers in cellular and infection microbiology · 2022Article
- A first insight into the genome of Prototheca wickerhamii, a major causative agent of human protothecosis.BMC genomics · 2021Article
- Protothecosis in Dogs and Cats-New Research Directions.Mycopathologia · 2021Review
- Slaying the last unicorn: discovery of histones in the microalgaRoyal Society open science · 2021Article
- Emergence of Fungal-Like Organisms: Prototheca.Mycopathologia · 2020Review
- Sequencing and Analysis of the Complete Organellar Genomes ofFrontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe complex cell wall structure of algae often precludes efficient extraction of their genetic material. The purpose of this study was to design a next-generation sequencing-suitable DNA isolation method for unicellular, achlorophyllous, yeast-like microalgae of the genus
resultsAll five, previously described, isolation assays yielded DNA concentrations lower than those obtained with the new method, averaging 16.15 ± 25.39 vs 74.2 ± 0.56 ng/µL, respectively. The new method was also superior in terms of DNA purity, as measured by A260/A280 (-0.41 ± 4.26 vs 2.02 ± 0.03), and A260/A230 (1.20 ± 1.12 vs 1.97 ± 0.07) ratios. Only the liquid nitrogen-based method yielded DNA of comparable quantity (60.96 ± 0.16 ng/µL) and quality (A260/A280 = 2.08 ± 0.02; A260/A230 = 2.23 ± 0.26). Still, the new method showed higher integrity, which was best illustrated upon electrophoretic analysis. Genomic DNA of
conclusionsA new method for DNA isolation from
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.