Evidence map›Paper›PMID 29025868›Full record

ArticleJournal of lipid research2017

In situ visualization of glucocerebrosidase in human skin tissue: zymography versus activity-based probe labeling.

Jeroen van Smeden, Irini M Dijkhoff, Richard W J Helder, Hanin Al-Khakany, Daphne E C Boer, Anne Schreuder, Wouter W Kallemeijn, Samira Absalah, Herman S Overkleeft, Johannes M F G Aerts and 1 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of lipid research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.6field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 17 citations in OpenAlex.

  1. DAPL1 is activated by Np63 and GRα and regulates lipid metabolism.Journal of molecular medicine (Berlin, Germany) · 2026
    Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Glycosphingolipids and Infection. Potential New Therapeutic Avenues.Frontiers in cell and developmental biology · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Jeroen van SmedenDivision of Drug Delivery Technology, Cluster Biotherapeutics, Leiden Academic Centre for Drug Research Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Irini M DijkhoffDivision of Drug Delivery Technology, Cluster Biotherapeutics, Leiden Academic Centre for Drug Research Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Richard W J HelderDivision of Drug Delivery Technology, Cluster Biotherapeutics, Leiden Academic Centre for Drug Research Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Hanin Al-KhakanyDivision of Drug Delivery Technology, Cluster Biotherapeutics, Leiden Academic Centre for Drug Research Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Daphne E C BoerMedical Biochemistry Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Anne SchreuderMedical Biochemistry Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Wouter W KallemeijnMedical Biochemistry Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.ORCID 0000-0002-3660-2930
Samira AbsalahDivision of Drug Delivery Technology, Cluster Biotherapeutics, Leiden Academic Centre for Drug Research Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Herman S OverkleeftDepartment of Bio-organic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.ORCID 0000-0001-6976-7005
Johannes M F G AertsMedical Biochemistry Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Joke A BouwstraDivision of Drug Delivery Technology, Cluster Biotherapeutics, Leiden Academic Centre for Drug Research Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands bouwstra@lacdr.leidenuniv.nl.
Leiden University · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Epidermal β-glucocerebrosidase (GBA1), an acid β-glucosidase normally located in lysosomes, converts (glucosyl)ceramides into ceramides, which is crucial to generate an optimal barrier function of the outermost skin layer, the stratum corneum (SC). Here we report on two developed in situ methods to localize active GBA in human epidermis:

Indexed as

Enzyme AssaysBenzoxazinesBoron CompoundsCyclohexanolsEpoxy CompoundsFluorescent DyesGene ExpressionGlucosidesGlucosylceramidaseHumansHymecromoneSkinStaining and LabelingTissue Culture Techniques4,4-difluoro-4-bora-3a,4a-diaza-s-indacene4-methylumbelliferyl glucosideBenzoxazinesBoron CompoundsCyclohexanolscyclophellitolEpoxy CompoundsFluorescent DyesGlucosidesGlucosylceramidaseHymecromoneresorufin glucopyranosideceramidesenzymology/enzyme regulationfluorescence microscopyGaucher diseasehuman skin equivalentsin situ zymographystratum corneumβ-glucocerebrosidase

Identifiers

PMID29025868
PMCPMC5711493
OpenAlexW2762650597

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.