ArticleBiochemistry2018
A Fluorescent Probe with Improved Water Solubility Permits the Analysis of Protein S-Depalmitoylation Activity in Live Cells.
Article in Biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Recent Overview of Protein Palmitoylation and Profiling Methodologies.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Recruitment, regulation, and release: Control of signaling enzyme localization and function by reversible S-acylation.The Journal of biological chemistry · 2024Review
- Depalmitoylation and cell physiology: APT1 as a mediator of metabolic signals.American journal of physiology. Cell physiology · 2024Review
- Regulation of ERK2 activity by dynamic S-acylation.Cell reports · 2023Article
- Activity-dependent post-translational regulation of palmitoylating and depalmitoylating enzymes in the hippocampus.Journal of cell science · 2023Article
- A High-Throughput Fluorescent Turn-On Assay for Inhibitors of DHHC Family Proteins.ACS chemical biology · 2022Article
- Cln5 represents a new type of cysteine-basedScience advances · 2022Article
- Inhibitors of DHHC family proteins.Current opinion in chemical biology · 2021Review
- Development of an Acrylamide-Based Inhibitor of ProteinACS chemical biology · 2021Article
- Quantitative Imaging of BiochemistryACS central science · 2020Review
- ABHD10 is an S-depalmitoylase affecting redox homeostasis through peroxiredoxin-5.Nature chemical biology · 2019Article
- Activity-Based Sensing ofAccounts of chemical research · 2019Review
- Measuring S-Depalmitoylation Activity In Vitro and In Live Cells with Fluorescent Probes.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging.ACS chemical biology · 2018Review
- Wnt5a signaling induced phosphorylation increases APT1 activity and promotes melanoma metastatic behavior.eLife · 2018Article
- Active and dynamic mitochondrial S-depalmitoylation revealed by targeted fluorescent probes.Nature communications · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
S-Palmitoylation is an abundant lipid post-translational modification that is dynamically installed on and removed from target proteins to regulate their activity and cellular localization. A dearth of tools for studying the activities and regulation of protein S-depalmitoylases, thioesterase "erasers" of protein cysteine S-palmitoylation, has contributed to an incomplete understanding of the role of dynamic S-palmitoylation in regulating proteome lipidation. Recently, we developed "depalmitoylation probes" (DPPs), small molecule probes that become fluorescent upon S-depalmitoylase enzymatic activity. To be suitable for application in live cells, the first-generation DPPs relied on a shorter lipid substrate (C8 vs naturally occurring C16), which enhanced solubility and cell permeability. However, the use of an unnatural lipid substrate on the probes potentially limits the utility of the approach. Herein, we present a new member of the DPP family, DPP-5, which features an anionic carboxylate functional group that increases the probe water solubility. The enhanced water solubility of DPP-5 permits the use of a natural, palmitoylated substrate (C16), rather than a surrogate lipid. We show that DPP-5 is capable of monitoring endogenous S-depalmitoylases in live mammalian cells and that it can reveal changes in S-depalmitoylation levels due to lipid stress. DPP-5 should prove to be a useful new tool for probing the regulation of proteome lipidation through dynamic S-depalmitoylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.