Evidence map›Paper›PMID 29022135›Full record

ArticleExtremophiles : life under extreme conditions2017

Pcal_1311, an alcohol dehydrogenase homologue from Pyrobaculum calidifontis, displays NADH-dependent high aldehyde reductase activity.

Raza Ashraf, Naeem Rashid, Tamotsu Kanai, Tadayuki Imanaka, Muhammad Akhtar

Abstract read
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In one paragraph

Article in Extremophiles : life under extreme conditions, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 3 countries.

Raza AshrafSchool of Biological Sciences, University of the Punjab, Lahore, 54590, Pakistan.
Naeem RashidSchool of Biological Sciences, University of the Punjab, Lahore, 54590, Pakistan. naeem.ff.sbs@pu.edu.pk.
Tamotsu KanaiDepartment of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto, 615-8510, Japan.
Tadayuki ImanakaThe Research Organization of Science and Technology, Ritsumeikan University, Kusatsu, Shiga, 525-8577, Japan.
Muhammad AkhtarSchool of Biological Sciences, University of the Punjab, Lahore, 54590, Pakistan.
University of the Punjab · PKKyoto Katsura Hospital · JPRitsumeikan University · JPUniversity of Southampton · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genome sequence of Pyrobaculum calidifontis, a hyperthermophilic archaeon, harbors three open-reading frames annotated as alcohol dehydrogenases. One of them, Pcal_1311, does not display a significantly high homology with any of the characterized alcohol dehydrogenases. Highest homology of 38% was found with the characterized counterpart from Geobacillus stearothermophilus. To examine the biochemical properties of Pcal_1311, we have cloned and functionally expressed the gene in Escherichia coli. Purified recombinant Pcal_1311 catalyzed the NAD(H)-dependent oxidation of various alcohols and reduction of aldehydes, with a marked preference for substrates with functional group at the terminal carbon. Highest activity for the oxidation reaction (3 μmol min

Indexed as

Alcohol DehydrogenaseAldehyde ReductaseBacterial ProteinsButylene GlycolsEnzyme StabilityGlutaralNADProtein DenaturationPyrobaculumSubstrate Specificity1,4-butanediolAlcohol DehydrogenaseAldehyde ReductaseBacterial ProteinsButylene GlycolsGlutaralNADAlcohol dehydrogenaseAldehyde reductaseCircular dichroismDenaturant tolerancePyrobaculum calidifontisThermostable

Identifiers

PMID29022135
OpenAlexW2761618231

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.