ArticleGigaScience2017
Development and validation of a multi-locus DNA metabarcoding method to identify endangered species in complex samples.
Article in GigaScience, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 111 citations in OpenAlex.
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- Towards real-time pork breed and boar taint classification using rapid evaporative ionisation mass spectrometry.NPJ science of food · 2026Article
- Cross-Platform Evaluation of Established NGS-Based Metabarcoding Methods for Detecting Food Fraud in Pistachio Products.Foods (Basel, Switzerland) · 2026Article
- An All-in-One Metabarcoding Approach to Mosquito and Arbovirus Xenosurveillance.Molecular ecology resources · 2025Article
- MitoCOMON: whole mitochondrial DNA sequencing by primer design and long overlapping amplicon assembly.BMC genomics · 2025Article
- Mass Spectrometric Fingerprinting to Detect Fraud and Herbal Adulteration in Plant Food Supplements.Molecules (Basel, Switzerland) · 2025Article
- The development and application of mini-barcodes from mitochondrial DNA for identifying medicinal leeches from traditional medicines.Scientific reports · 2025Article
- Authentication of the Botanical Origin of Honey:Journal of agricultural and food chemistry · 2025Article
- Authenticity in bakery products: Detection of pistachio fraud using NGS metabarcoding.Food chemistry. Molecular sciences · 2025Article
- Protein Coding Low-CopyEcology and evolution · 2025Article
- Improving the profiling of wheat bacterial and fungal endophytic communities-a PCR clamping approach.Frontiers in microbiology · 2025Article
- DNA metabarcoding unveils authenticity and adulteration in commercial Chinese polyherbal preparations: Renshen Jianpi Wan as a critical case study.Frontiers in pharmacology · 2025Article
- Integrating DNA Barcoding Within an Orthogonal Approach for Herbal Product Authentication: A Narrative Review.Phytochemical analysis : PCA · 2025Review
- Article
- Decoding Seafood: Multi-Marker Metabarcoding for Authenticating Processed Seafood.Foods (Basel, Switzerland) · 2024Article
- Long-term co-circulation of multiple arboviruses in southeast Australia revealed by xeno-monitoring and viral whole-genome sequencing.Virus evolution · 2024Article
- Three-tiered authentication of herbal traditional Chinese medicine ingredients used in women's health provides progressive qualitative and quantitative insight.Frontiers in pharmacology · 2024Article
- Validating a multi-locus metabarcoding approach for characterizing mixed-pollen samples.Plant methods · 2023Article
Corrections and comments
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Authors and funding
29 authors at 9 institutions in 8 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA metabarcoding provides great potential for species identification in complex samples such as food supplements and traditional medicines. Such a method would aid Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) enforcement officers to combat wildlife crime by preventing illegal trade of endangered plant and animal species. The objective of this research was to develop a multi-locus DNA metabarcoding method for forensic wildlife species identification and to evaluate the applicability and reproducibility of this approach across different laboratories. A DNA metabarcoding method was developed that makes use of 12 DNA barcode markers that have demonstrated universal applicability across a wide range of plant and animal taxa and that facilitate the identification of species in samples containing degraded DNA. The DNA metabarcoding method was developed based on Illumina MiSeq amplicon sequencing of well-defined experimental mixtures, for which a bioinformatics pipeline with user-friendly web-interface was developed. The performance of the DNA metabarcoding method was assessed in an international validation trial by 16 laboratories, in which the method was found to be highly reproducible and sensitive enough to identify species present in a mixture at 1% dry weight content. The advanced multi-locus DNA metabarcoding method assessed in this study provides reliable and detailed data on the composition of complex food products, including information on the presence of CITES-listed species. The method can provide improved resolution for species identification, while verifying species with multiple DNA barcodes contributes to an enhanced quality assurance.
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