Evidence map›Paper›PMID 29020119›Full record

ArticlePloS one2017

Human adipose-derived stem cells support the growth of limbal stem/progenitor cells.

Hua Mei, Sheyla González, Martin N Nakatsu, Elfren R Baclagon, Felix V Chen, Sophie X Deng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact, top 100% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 0 citations in OpenAlex.

  1. Characterization of the Limbal Epithelial Stem Cell Niche.Investigative ophthalmology & visual science · 2023
    Article
  2. Review
  3. Concise Review: Bioengineering of Limbal Stem Cell Niche.Bioengineering (Basel, Switzerland) · 2023
    Review
  4. Review
  5. Article
  6. Article
  7. Review
  8. Review
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Hua MeiCornea Division, Stein Eye Institute, University of California, Los Angeles, California, United States of America.
Sheyla GonzálezCornea Division, Stein Eye Institute, University of California, Los Angeles, California, United States of America.
Martin N NakatsuCornea Division, Stein Eye Institute, University of California, Los Angeles, California, United States of America.
Elfren R BaclagonCornea Division, Stein Eye Institute, University of California, Los Angeles, California, United States of America.
Felix V ChenCornea Division, Stein Eye Institute, University of California, Los Angeles, California, United States of America.
Sophie X DengCornea Division, Stein Eye Institute, University of California, Los Angeles, California, United States of America.
University of California, Los Angeles · US

Funding

Vision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Xian-Jie Yang · 1985 to 2026
$16.8M
Ex Vivo Expansion of Human Limbal Stem Cells for TransplantationR01EY021797 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Sophie Deng · 2012 to 2026
$5.1M
NEI NIH HHS P30 EY000331NEI NIH HHS R01 EY021797
6 · The paper itself

Abstract

The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells. To reduce possible xenobiotic contamination from 3T3s, primary human adipose-derived stem cells (ASCs) were examined as feeder cells to support the expansion of LSCs in vitro. To optimize the ASC-supported culture, freshly isolated limbal epithelial cells in the form of single cells (SC-ASC) or cell clusters (CC-ASC) were cultured using three different methods: LSCs seeded directly on feeder cells, a 3-dimensional (3D) culture system and a 3D culture system with fibrin (fibrin 3D). The expanded LSCs were examined at the end of a 2-week culture. The standard 3T3 culture served as control. Expansion of SC-ASC showed limited proliferation and exhibited differentiated morphology. CC-ASC generated epithelial cells with undifferentiated morphology in all culture methods, among which CC-ASC in 3D culture supported the highest cell doubling (cells doubled 9.0 times compared to cells doubled 4.9 times in control) while maintained the percentage of putative limbal stem/progenitor cells compared to the control. There were few cell-cell contacts between cultured LSCs and ASCs in 3D CC-ASC. In conclusion, ASCs support the growth of LSCs in the form of cell clusters but not in single cells. 3D CC-ASC could serve as a substitute for the standard 3T3 culture to expand LSCs.

Indexed as

3T3 CellsAdipose TissueAdultAgedAnimalsBiomarkersCell AggregationCell CommunicationCell CountCell DifferentiationCell ProliferationCells, CulturedEpithelial CellsFeeder CellsHumansImmunohistochemistryBiomarkersRNA, MessengerTP63 protein, humanTranscription FactorsTumor Suppressor Proteins

Identifiers

PMID29020119
PMCPMC5636133
OpenAlexW827878460

What OpenQuestion holds

Textmetadata
LicenceCC0
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.