ArticleFrontiers in plant science2017
Template Preparation Affects 16S rRNA High-Throughput Sequencing Analysis of Phyllosphere Microbial Communities.
Article in Frontiers in plant science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Common garden experiments suggest terpene-mediated associations between phyllosphere microbes and Japanese cedar.Scientific reports · 2025Article
- Phyllosphere of Agathis australis Leaves and the Impact of the Soil-Borne Pathogen Phytophthora agathidicida.Microbial ecology · 2024Article
- Response of Phyllosphere and Rhizosphere Microbial Communities to Salt Stress ofPlants (Basel, Switzerland) · 2024Article
- The Greenhouse Phyllosphere Microbiome and Associations with Introduced Bumblebees and Predatory Mites.Microbiology spectrum · 2022Article
- Cytokinin-microbiome interactions regulate developmental functions.Environmental microbiome · 2022Article
- Cytokinin drives assembly of the phyllosphere microbiome and promotes disease resistance through structural and chemical cues.The ISME journal · 2022Article
- Dominant egg surface bacteria of Holotrichia oblita (Coleoptera: Scarabaeidae) inhibit the multiplication of Bacillus thuringiensis and Beauveria bassiana.Scientific reports · 2021Article
- Standardization of Plant Microbiome Studies: Which Proportion of the Microbiota is Really Harvested?Microorganisms · 2020Article
- Niche Differentiation in the Composition, Predicted Function, and Co-occurrence Networks in Bacterial Communities Associated With Antarctic Vascular Plants.Frontiers in microbiology · 2020Article
- Guanidine thiocyanate solution facilitates sample collection for plant rhizosphere microbiome analysis.PeerJ · 2019Article
- Effect of Different Disinfectants on Bacterial Aerosol Diversity in Poultry Houses.Frontiers in microbiology · 2018Article
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8 authors.
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Abstract
Phyllosphere microbial communities are highly diverse and have important ecological implications; in that context, bacterial identification based on 16S rRNA genes is an important research issue. In studies of phyllosphere microbial communities, microporous filtration and centrifugation are used to collect microorganism samples, but it is unclear which one has a better collection efficiency. In this study, we compared these two microorganism collection methods and investigated the effects of the DNA extraction process on the estimation of microbial community composition and organization. The following four treatments were examined: (A) filtration, resuspension, and direct PCR; (B) filtration, DNA isolation, and PCR; (C) centrifugation, resuspension, and direct PCR; (D) centrifugation, DNA isolation, and PCR. Our results showed that the percentage of chloroplast sequence contaminants was affected by the DNA extraction process. The bacterial compositions clearly differed between treatments A and C, suggesting that the collection method has an influence on the determination of community structure. Compared with treatments B and D, treatments A and C resulted in higher Shannon index values, indicating that the DNA extraction process might reduce the observed phyllosphere microbial alpha diversity. However, with respect to community structure, treatments B and D yielded very similar results, suggesting that the DNA extraction process erases the effect of the collection method. Our findings provide key information to ensure accurate estimates of diversity and community composition in studies of phyllosphere microorganisms.
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