ArticleFrontiers in plant science2017
Sequencing of Single Pollen Nuclei Reveals Meiotic Recombination Events at Megabase Resolution and Circumvents Segregation Distortion Caused by Postmeiotic Processes.
Article in Frontiers in plant science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Mapping recombination cold spots in wheat via meiotic recombination in a large biparental population.G3 (Bethesda, Md.) · 2026Article
- A simple and high-throughput single pollen genotyping pipeline to study meiosis in apple (Malus x domestica).Plant methods · 2026Article
- Population-wide single-pollen nuclei genotyping in rye sheds light on the genetic basis and environmental plasticity of meiotic recombination.The New phytologist · 2026Article
- Crystal Digital PCR™-Based Single Nucleus Pollen Genotyping in Barley (Hordeum vulgare L.) to Measure Meiotic Crossover Rates in Defined Chromosomal Intervals.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- Plant sperm cell sequencing for genome phasing and determination of meiotic crossover points.Nature protocols · 2025Review
- A Simple and User-Friendly Method for High-Quality Preparation of Pollen Grains for Scanning Electron Microscopy (SEM).Plants (Basel, Switzerland) · 2024Article
- The Control of the Crossover Localization in Allium.International journal of molecular sciences · 2023Article
- Article
- Wild barley (Hordeum spontaneum) and landraces (Hordeum vulgare) from Turkey contain an abundance of novel Rhynchosporium commune resistance loci.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- A technical guide to TRITEX, a computational pipeline for chromosome-scale sequence assembly of plant genomes.Plant methods · 2022Article
- Measuring the frequency and distribution of meiotic crossovers in homozygous barley inbred lines.Frontiers in plant science · 2022Article
- Applications of Single-Cell DNA Sequencing.Annual review of genomics and human genetics · 2021Review
- High-Frequency Homologous Recombination Occurred Preferentially inFrontiers in genetics · 2021Article
- Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering.Nature communications · 2020Article
- From molecules to populations: appreciating and estimating recombination rate variation.Nature reviews. Genetics · 2020Review
- Analysis of Crossover Events and Allele Segregation Distortion in Interspecific Citrus Hybrids by Single Pollen Genotyping.Frontiers in plant science · 2020Article
- More Filtering on SNP Calling Does Not Remove Evidence of Inter-Nucleus Recombination in Dikaryotic Arbuscular Mycorrhizal Fungi.Frontiers in plant science · 2020Article
- Crossover Interference: Shedding Light on the Evolution of Recombination.Annual review of genetics · 2019Review
- Linked-read sequencing of gametes allows efficient genome-wide analysis of meiotic recombination.Nature communications · 2019Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Meiotic recombination is a fundamental mechanism to generate novel allelic combinations which can be harnessed by breeders to achieve crop improvement. The recombination landscape of many crop species, including the major crop barley, is characterized by a dearth of recombination in 65% of the genome. In addition, segregation distortion caused by selection on genetically linked loci is a frequent and undesirable phenomenon in double haploid populations which hampers genetic mapping and breeding. Here, we present an approach to directly investigate recombination at the DNA sequence level by combining flow-sorting of haploid pollen nuclei of barley with single-cell genome sequencing. We confirm the skewed distribution of recombination events toward distal chromosomal regions at megabase resolution and show that segregation distortion is almost absent if directly measured in pollen. Furthermore, we show a bimodal distribution of inter-crossover distances, which supports the existence of two classes of crossovers which are sensitive or less sensitive to physical interference. We conclude that single pollen nuclei sequencing is an approach capable of revealing recombination patterns in the absence of segregation distortion.
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