ArticleScientific reports2017
Difference in intracellular temperature rise between matured and precursor brown adipocytes in response to uncoupler and β-adrenergic agonist stimuli.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
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- Thermochromic luminescence of a π-conjugated polymer based on boron pyridylenolate complex.Scientific reports · 2025Article
- Clinical Applications of Adipose-Derived Stem Cell (ADSC) Exosomes in Tissue Regeneration.International journal of molecular sciences · 2024Review
- Warm Cells, Hot Mitochondria: Achievements and Problems of Ultralocal Thermometry.International journal of molecular sciences · 2023Review
- Measurement of Intracellular Temperature in Brown Adipocytes Using a Cationic Fluorescent Polymeric Thermometer.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Polymeric Nanoparticles with Embedded Eu(III) Complexes as Molecular Probes for Temperature Sensing.Molecules (Basel, Switzerland) · 2022Article
- How sticky? How tight? How hot? Imaging probes for fluid viscosity, membrane tension and temperature measurements at the cellular level.The international journal of biochemistry & cell biology · 2022Review
- Uncoupling Protein 1 Does Not Produce Heat without Activation.International journal of molecular sciences · 2022Review
- Intracellular thermometry uncovers spontaneous thermogenesis and associated thermal signaling.Communications biology · 2021Review
- Adipose-derived stromal cells for nonhealing wounds: Emerging opportunities and challenges.Medicinal research reviews · 2021Review
- Advances and challenges for fluorescence nanothermometry.Nature methods · 2020Review
- A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature.Nanoscale advances · 2020Article
- A Personal Journey across Fluorescent Sensing and Logic Associated with Polymers of Various Kinds.Polymers · 2019Review
- Article
- GFP fluorescence peak fraction analysis based nanothermometer for the assessment of exothermal mitochondria activity in live cells.Scientific reports · 2019Article
- Monitoring spin coherence of single nitrogen-vacancy centers in nanodiamonds during pH changes in aqueous buffer solutions.RSC advances · 2019Article
- Temperature imaging using a cationic linear fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy.Nature protocols · 2019Article
- Transient heat release during induced mitochondrial proton uncoupling.Communications biology · 2019Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brown adipocytes function to maintain body temperature by heat production. However, direct measurement of heat production at a single cell level remains difficult. Here we developed a method to measure the temperature within primary cultured brown adipocytes using a cationic fluorescent polymeric thermometer. Placement of the thermometer within a matured brown adipocyte and a precursor cell enabled the detection of heat production following uncoupler treatment. The increase in the intracellular temperature due to stimulation with a mitochondrial uncoupler was higher in matured brown adipocytes than in precursor cells. Stimulation with a β-adrenergic receptor (β-AR) agonist, norepinephrine, raised the intracellular temperature of matured brown adipocytes to a level comparable to that observed after stimulation with a β3-AR-specific agonist, CL316.243. In contrast, neither β-AR agonist induced an intracellular temperature increase in precursor cells. Further, pretreatment of brown adipocytes with a β3-AR antagonist inhibited the norepinephrine-stimulated elevation of temperature. These results demonstrate that our novel method successfully determined the difference in intracellular temperature increase between matured brown adipocytes and precursor cells in response to stimulation by an uncoupler and β-AR agonists.
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