Evidence map›Paper›PMID 29017607›Full record

ArticleMicrobiome2017

Non-digestible oligosaccharides directly regulate host kinome to modulate host inflammatory responses without alterations in the gut microbiota.

Richard Y Wu, Pekka Määttänen, Scott Napper, Erin Scruten, Bo Li, Yuhki Koike, Kathene C Johnson-Henry, Agostino Pierro, Laura Rossi, Steven R Botts and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiome, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed, 1 pooled it
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 1 synthesis or guideline pooled it, 78 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 1 country.

Richard Y WuCell Biology Program, Research Institute, Division of Gastroenterology, Hepatology and Nutrition, Hospital for Sick Children, 555 University Avenue, Toronto, ON, M5G 1X8, Canada.
Pekka MäättänenCell Biology Program, Research Institute, Division of Gastroenterology, Hepatology and Nutrition, Hospital for Sick Children, 555 University Avenue, Toronto, ON, M5G 1X8, Canada.
Scott NapperVaccine and Infectious Disease Organization-International Vaccine Center, University of Saskatchewan, Saskatoon, SK, Canada.
Erin ScrutenVaccine and Infectious Disease Organization-International Vaccine Center, University of Saskatchewan, Saskatoon, SK, Canada.
Bo LiPhysiology and Experimental Medicine, Research Institute, Hospital for Sick Children, Toronto, ON, Canada.
Yuhki KoikePhysiology and Experimental Medicine, Research Institute, Hospital for Sick Children, Toronto, ON, Canada.
Kathene C Johnson-HenryCell Biology Program, Research Institute, Division of Gastroenterology, Hepatology and Nutrition, Hospital for Sick Children, 555 University Avenue, Toronto, ON, M5G 1X8, Canada.
Agostino PierroPhysiology and Experimental Medicine, Research Institute, Hospital for Sick Children, Toronto, ON, Canada.
Laura RossiDepartment of Medicine, Faculty of Health Sciences, McMaster University, Hamilton, ON, Canada.
Steven R BottsCell Biology Program, Research Institute, Division of Gastroenterology, Hepatology and Nutrition, Hospital for Sick Children, 555 University Avenue, Toronto, ON, M5G 1X8, Canada.
Michael G SuretteDepartment of Medicine, Faculty of Health Sciences, McMaster University, Hamilton, ON, Canada.
Philip M ShermanCell Biology Program, Research Institute, Division of Gastroenterology, Hepatology and Nutrition, Hospital for Sick Children, 555 University Avenue, Toronto, ON, M5G 1X8, Canada. philip.sherman@sickkids.ca.
Hospital for Sick Children · CAMcMaster University · CAUniversity of Saskatchewan · CABurman University · CAUniversity of Toronto · CA

Funding

CIHR IOP-92890CIHR MOP-89894
6 · The paper itself

Abstract

backgroundPrebiotics are non-digestible food ingredients that enhance the growth of certain microbes within the gut microbiota. Prebiotic consumption generates immune-modulatory effects that are traditionally thought to reflect microbial interactions within the gut. However, recent evidence suggests they may also impart direct microbe-independent effects on the host, though the mechanisms of which are currently unclear.

methodsKinome arrays were used to profile the host intestinal signaling responses to prebiotic exposures in the absence of microbes. Identified pathways were functionally validated in Caco-2Bbe1 intestinal cell line and in vivo model of murine endotoxemia.

resultsWe found that prebiotics directly regulate host mucosal signaling to alter response to bacterial infection. Intestinal epithelial cells (IECs) exposed to prebiotics are hyporesponsive to pathogen-induced mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) activations, and have a kinome profile distinct from non-treated cells pertaining to multiple innate immune signaling pathways. Consistent with this finding, mice orally gavaged with prebiotics showed dampened inflammatory response to lipopolysaccharide (LPS) without alterations in the gut microbiota.

conclusionsThese findings provide molecular mechanisms of direct host-prebiotic interactions to support prebiotics as potent modulators of host inflammation.

Indexed as

Gastrointestinal MicrobiomeInflammationPrebioticsAnimalsCaco-2 CellsEndotoxemiaHumansImmunity, InnateIntestinal MucosaLipopolysaccharidesMiceMitogen-Activated Protein Kinase KinasesNF-kappaB-Inducing KinaseOligosaccharidesProtein Array AnalysisProtein KinasesLipopolysaccharidesMitogen-Activated Protein Kinase KinasesNF-kappaB-Inducing KinaseOligosaccharidesPrebioticsProtein KinasesProtein Serine-Threonine KinasesE. coliKinomeLipopolysaccharideNon-digestible oligosaccharidesPrebiotics

Identifiers

PMID29017607
PMCPMC5635512
OpenAlexW2764046617

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.