ArticleParasites & vectors2017
Evidence for viable and stable triploid Trypanosoma congolense parasites.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- Ancestral aneuploidy and stable chromosomal duplication resulting in differential genome structure and gene expression control in trypanosomatid parasites.Genome research · 2024Article
- Genome plasticity driven by aneuploidy and loss of heterozygosity inMicrobial genomics · 2022Article
- Optimal reference genes for gene expression analysis in polyploid of Cyprinus carpio and Carassius auratus.BMC genetics · 2020Article
- Meiotic sex in Chagas disease parasite Trypanosoma cruzi.Nature communications · 2019Article
- Enhancing vector refractoriness to trypanosome infection: achievements, challenges and perspectives.BMC microbiology · 2018Article
- Chromosomal copy number variation analysis by next generation sequencing confirms ploidy stability in Trypanosoma brucei subspecies.Microbial genomics · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
backgroundRecent whole genome sequencing (WGS) analysis identified a viable triploid strain of Trypanosoma congolense. This triploid strain BANANCL2 was a clone of the field isolate BANAN/83/CRTRA/64 that was collected from cattle in Burkina Faso in 1983.
resultsWe demonstrated the viability and stability of triploidy throughout the complete life-cycle of the parasite by infecting tsetse flies with the triploid clone BANANCL2. Proboscis-positive tsetse flies efficiently transmitted the parasites to mice resulting in systemic infections. WGS of the parasites was performed at all life-cycle stages, and a method based on a block alternative allele frequency spectrum was developed to efficiently detect the ploidy profiles of samples with low read depth. This approach confirmed the triploid profile of parasites throughout their life-cycle in the tsetse fly and the mammalian host, demonstrating that triploidy is present at all stages and is stable over time.
conclusionThe presence of viable field-isolated triploid parasites indicates another possible layer of genetic diversity in natural T. congolense populations. The comparison between triploid and diploid parasites provides a unique model system to study the impact of chromosome copy number variations in African trypanosomes. In addition, the consequences of triploidy can be further investigated using this stable triploid model.
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