ArticlePLoS pathogens2017
Targeted mutagenesis in a human-parasitic nematode.
Article in PLoS pathogens, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed.
- Transgenic hookworm secretes anti-tetrodotoxin human single chain antibody.Nature communications · 2026Article
- Neuronally sensed oxygen drives behavior and development in human-infective, skin-penetrating nematodes.bioRxiv : the preprint server for biology · 2026Article
- Transgenesis and targeted mutagenesis in the human-parasitic nematodeFrontiers in parasitology · 2026Review
- Unearthing roundworms: Nematodes as determinants of human health.One health (Amsterdam, Netherlands) · 2025Review
- CRISPR/Cas genome editing, functional genomics, and diagnostics for parasitic helminths.International journal for parasitology · 2025Review
- Aptazyme-mediated gene regulation in Strongyloides stercoralis for functional studies of insulin receptor isoform specificity.PLoS pathogens · 2025Article
- Dopamine signaling drives skin invasion by human-infective nematodes.Nature communications · 2025Article
- Advances in Anthelmintic Target Identification.International journal of molecular sciences · 2025Review
- Viruses of parasites: A roadmap toward diagnostic and therapeutic development.PLoS neglected tropical diseases · 2025Review
- Current trends in application of CRISPR/Cas9 in gene editing and diagnostics in Neglected tropical diseases (NTDs).Molecular biology reports · 2025Review
- Carbon dioxide shapes parasite-host interactions in a human-infective nematode.Current biology : CB · 2025Article
- Applications of CRISPR in Parasitology.Current pharmaceutical biotechnology · 2025Review
- Unraveling the microRNAs Involved in Fasciolosis: Master Regulators of the Host-Parasite Crosstalk.International journal of molecular sciences · 2024Review
- The generation of stable transgenic lines in the human-infective nematode Strongyloides stercoralis.G3 (Bethesda, Md.) · 2024Article
- Systemic Immune Modulation by Gastrointestinal Nematodes.Annual review of immunology · 2024Review
- Carbon dioxide shapes parasite-host interactions in a human-infective nematode.bioRxiv : the preprint server for biology · 2024Article
- Progresses and challenges inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Invade or die: behaviours and biochemical mechanisms that drive skin penetration inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Review
- A standard workflow for community-driven manual curation ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
Abstract
Parasitic nematodes infect over 1 billion people worldwide and cause some of the most common neglected tropical diseases. Despite their prevalence, our understanding of the biology of parasitic nematodes has been limited by the lack of tools for genetic intervention. In particular, it has not yet been possible to generate targeted gene disruptions and mutant phenotypes in any parasitic nematode. Here, we report the development of a method for introducing CRISPR-Cas9-mediated gene disruptions in the human-parasitic threadworm Strongyloides stercoralis. We disrupted the S. stercoralis twitchin gene unc-22, resulting in nematodes with severe motility defects. Ss-unc-22 mutations were resolved by homology-directed repair when a repair template was provided. Omission of a repair template resulted in deletions at the target locus. Ss-unc-22 mutations were heritable; we passed Ss-unc-22 mutants through a host and successfully recovered mutant progeny. Using a similar approach, we also disrupted the unc-22 gene of the rat-parasitic nematode Strongyloides ratti. Our results demonstrate the applicability of CRISPR-Cas9 to parasitic nematodes, and thereby enable future studies of gene function in these medically relevant but previously genetically intractable parasites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.