ArticleJournal of visualized experiments : JoVE2017
Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Decoding Sulfur-Containing Aroma Compounds in Foods: From Key Odorant Mapping to Structure-Odor Mechanisms and Flavor Design.Foods (Basel, Switzerland) · 2026Review
- Allosteric Gating Mechanism Regulates Odorant Selectivity and Antagonism in Odorant Receptors.bioRxiv : the preprint server for biology · 2026Article
- Rapid odorant metabolism organizes identity- and timing-based odor representations by olfactory bulb inputs and outputs.bioRxiv : the preprint server for biology · 2026Article
- Roles of sensory receptors in non-sensory organs: the kidney and beyond.Nature reviews. Nephrology · 2025Review
- Identification of Olfactory Receptors Responding to Androstenone and the Key Structure Determinant in Domestic Pig.Current issues in molecular biology · 2024Article
- Article
- Intracellular Allosteric Antagonist of the Olfactory Receptor OR51E2.Molecular pharmacology · 2024Article
- A chemical signal in human female tears lowers aggression in males.PLoS biology · 2023Article
- Engineered odorant receptors illuminate structural principles of odor discrimination.bioRxiv : the preprint server for biology · 2023Article
- The Luminescence Hypothesis of Olfaction.Sensors (Basel, Switzerland) · 2023Article
- Odor Discrimination by Lipid Membranes.Membranes · 2023Article
- Modulation of the combinatorial code of odorant receptor response patterns in odorant mixtures.Molecular and cellular neurosciences · 2020Article
- Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase.Journal of visualized experiments : JoVE · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The enormous sizes of the mammalian odorant receptor (OR) families present difficulties to find their cognate ligands among numerous volatile chemicals. To efficiently and accurately deorphanize ORs, we combine the use of a heterologous cell line to express mammalian ORs and a genetically modified biosensor plasmid to measure cAMP production downstream of OR activation in real time. This assay can be used to screen odorants against ORs and vice versa. Positive odorant-receptor interactions from the screens can be subsequently confirmed by testing against various odor concentrations, generating concentration-response curves. Here we used this method to perform a high-throughput screening of an odorous compound against a human OR library expressed in Hana3A cells and confirmed that the positively-responding receptor is the cognate receptor for the compound of interest. We found this high-throughput detection method to be efficient and reliable in assessing OR activation and our data provide an example of its potential use in OR functional studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.