ArticleJournal of visualized experiments : JoVE2017
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Limited transmission of cervid prions to nonhuman primates provides insights into the zoonotic potential of chronic wasting disease.Science advances · 2026Article
- Comparison of acetone and sodium phosphotungstic acid precipitation for sample enrichment prior to RT-QuIC for the detection of prion disease.BMC research notes · 2026Article
- Prions and protein aggregates as pathogens, self-propagating structures, biomarkers, and therapeutic targets.Microbiology and molecular biology reviews : MMBR · 2025Review
- RT-QuIC detection of chronic wasting disease prions in third eyelids from white-tailed deer.Scientific reports · 2025Article
- Temporal Characterization of Prion Shedding in Secreta of White-Tailed Deer in Longitudinal Study of Chronic Wasting Disease, United States.Emerging infectious diseases · 2024Article
- Norwegian moose CWD induces clinical disease and neuroinvasion in gene-targeted mice expressing cervid S138N prion protein.PLoS pathogens · 2024Article
- Article
- Dynamics of CWD prion detection in feces and blood from naturally infected white-tailed deer.Scientific reports · 2023Article
- Propagation of PrPCommunications biology · 2023Article
- Heterozygosity for cervid S138N polymorphism results in subclinical CWD in gene-targeted mice and progressive inhibition of prion conversion.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Development of a sensitive real-time quaking-induced conversion (RT-QuIC) assay for application in prion-infected blood.PloS one · 2023Article
- Review
- Clinical application of prion-like seeding in α-synucleinopathies: Early and non-invasive diagnosis and therapeutic development.Frontiers in molecular neuroscience · 2022Review
- Detection of chronic wasting disease in feces and recto-anal mucosal associated lymphoid tissues with RT-QuIC in a naturally infected farmed white-tailed deer herd.Frontiers in veterinary science · 2022Article
- New and distinct chronic wasting disease strains associated with cervid polymorphism at codon 116 of the Prnp gene.PLoS pathogens · 2021Article
- Real-Time Quaking-Induced Conversion Detection of PrPFrontiers in veterinary science · 2021Article
- Large-scale prion protein genotyping in Canadian caribou populations and potential impact on chronic wasting disease susceptibility.Molecular ecology · 2020Article
- Proteopathic Seed Amplification Assays for Neurodegenerative Disorders.Clinics in laboratory medicine · 2020Review
- Detection of Pathognomonic Biomarker PrPBiomolecules · 2020Review
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RT-QuIC technique is a sensitive in vitro cell-free prion amplification assay based mainly on the seeded misfolding and aggregation of recombinant prion protein (PrP) substrate using prion seeds as a template for the conversion. RT-QuIC is a novel high-throughput technique which is analogous to real-time polymerase chain reaction (PCR). Detection of amyloid fibril growth is based on the dye Thioflavin T, which fluoresces upon specific interaction with ᵦ-sheet rich proteins. Thus, amyloid formation can be detected in real time. We attempted to develop a reliable non-invasive screening test to detect chronic wasting disease (CWD) prions in fecal extract. Here, we have specifically adapted the RT-QuIC technique to reveal PrP
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.