Evidence map›Paper›PMID 28993918›Full record

ArticleClinical oral investigations2018

A new albumin-depletion strategy improves proteomic research of gingival crevicular fluid from periodontitis patients.

Sarah Batschkus, Goekhan Cingoez, Henning Urlaub, Nicolai Miosge, Christian Kirschneck, Philipp Meyer-Marcotty, Christof Lenz

Abstract read
PubMed Publisher
In one paragraph

Article in Clinical oral investigations, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Sarah BatschkusDepartment for Orthodontics, Center for Dentistry, Oral Medicine and Craniomaxillofacial Surgery, University Medical Center, Georg August University, Robert-Koch-Str. 40, 37075, Göttingen, Germany. sarah.batschkus@med.uni-goettingen.de.ORCID http://orcid.org/0000-0002-0656-6537
Goekhan CingoezDepartment for Orthodontics, Center for Dentistry, Oral Medicine and Craniomaxillofacial Surgery, University Medical Center, Georg August University, Robert-Koch-Str. 40, 37075, Göttingen, Germany.
Henning UrlaubMax Planck Institute for Biophysical Chemistry, Bioanalytical Mass Spectrometry Group, Am Fassberg 11, 37077, Goettingen, Germany.
Nicolai MiosgeTissue Regeneration Group, Department of Prosthodontics, University Medical Center Goettingen, Robert-Koch-Straße 40, 37075, Goettingen, Germany.
Christian KirschneckDepartment of Orthodontics, University Medical Center Regensburg, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany.
Philipp Meyer-MarcottyDepartment for Orthodontics, Center for Dentistry, Oral Medicine and Craniomaxillofacial Surgery, University Medical Center, Georg August University, Robert-Koch-Str. 40, 37075, Göttingen, Germany.
Christof LenzMax Planck Institute for Biophysical Chemistry, Bioanalytical Mass Spectrometry Group, Am Fassberg 11, 37077, Goettingen, Germany.
University of Göttingen · DEMax Planck Institute for Biophysical Chemistry · DEUniversity Hospital Regensburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesGingival crevicular fluid (GCF), the inflammatory infiltrate within the crevicular sulcus, is of great importance for diverse processes in the oral cavity and has a high impact in oral sciences. It is assumed to serve as a source of biomarkers for systemic or periodontal diseases and mediators of orthodontic tooth movement. In order to characterize the protein content of the GCF in an unbiased and complete approach, we employed mass spectrometry (MS), which allows not only the identification, but also the quantification of these proteins. In samples obtained from patients suffering from periodontitis, this method is often limited due to the presence of highly abundant serum albumin deriving from serum. The aim of this investigation was to employ a protein precipitation procedure for the efficient depletion of serum albumin from GCF samples. MATERIALS AND

methodsGFC samples collected from five adult periodontitis patients were fractionated by trichloroacetic acid/acetone precipitation and the resulting soluble and pelleted fractions were analyzed by SDS-PAGE and high-resolution mass spectrometry.

resultsTrichloroacetic acid/acetone precipitation was successfully employed as a protein precipitation procedure for the efficient depletion of serum albumin from GCF samples. Careful analysis revealed that the precipitation step reduced the serum albumin content efficiently, and increased subsequent protein identifications by 32%. Three hundred seventeen proteins could only be identified with this new approach.

conclusionThe increased coverage of the GCF proteome will help improve our understanding of molecular mechanisms in the periodontium during pathogenesis of periodontitis. CLINICAL RELEVANCE: Our new albumin depletion strategy combined with high-resolution mass spectrometry can be used to effectively monitor the molecular signals of the periodontium.

Indexed as

ProteomicsAdultBiomarkersElectrophoresis, Polyacrylamide GelGingival Crevicular FluidHumansMass SpectrometryPeriodontitisSerum AlbuminBiomarkersSerum AlbuminGingival crevicular fluidMass spectrometryOrthodontic tooth movementPeriodontitisSerum albumin

Identifiers

PMID28993918
OpenAlexW2762805868

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.