Evidence map›Paper›PMID 28993251›Full record

ArticleExperimental neurology2018

Hypoxia-ischemia modifies postsynaptic GluN2B-containing NMDA receptor complexes in the neonatal mouse brain.

Fuxin Lu, Guo Shao, Yongqiang Wang, Shenheng Guan, Alma L Burlingame, Xuemei Liu, Xiao Liang, Renatta Knox, Donna M Ferriero, Xiangning Jiang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental neurology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.6field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Fuxin LuDepartment of Pediatrics, University of California San Francisco, CA, USA.
Guo ShaoInner Mongolia Key Laboratory of Hypoxic Translational Medicine, Baotou Medical College, Baotou, China.
Yongqiang WangDepartment of Cellular & Molecular Pharmacology, University of California San Francisco, CA, USA; Howard Hughes Medical Institute, University of California, San Francisco, CA, USA.
Shenheng GuanDepartment of Pharmaceutical Chemistry, University of California San Francisco, CA, USA.
Alma L BurlingameDepartment of Pharmaceutical Chemistry, University of California San Francisco, CA, USA.
Xuemei LiuCentral Laboratory, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China.
Xiao LiangCentral Laboratory, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China.
Renatta KnoxDepartment of Pediatrics, Weill Cornell Medical College, New York, NY, USA.
Donna M FerrieroDepartment of Pediatrics, University of California San Francisco, CA, USA; Department of Neurology, University of California San Francisco, CA, USA.
Xiangning JiangDepartment of Pediatrics, University of California San Francisco, CA, USA. Electronic address: xiangning.jiang@ucsf.edu.
University of California, San Francisco · USBeijing University of Chinese Medicine · CNBaotou Medical College · CNCornell University · USHoward Hughes Medical Institute · US

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM007618 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ANDERSON, MARK S · 1985 to 2020
$29.6M
Bio-Organic Biomedical Mass Spectromy ResourceP41GM103481 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BURLINGAME, ALMA L · 2012 to 2015
$6.4M
Precision Therapy for Neonatal Brain InjuryR35NS097299 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIERO, DONNA M. · 2017 to 2024
$6.3M
Oxidant Mechanisms in Neonatal Brain InjuryR01NS033997 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIERO, DONNA M. · 1996 to 2016
$5.1M
Phospho-regulation of NMDA receptors in neonatal brain hypoxia-ischemiaR01NS084057 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JIANG, XIANGNING · 2014 to 2018
$1.7M
NIGMS NIH HHS P41 GM103481NIGMS NIH HHS T32 GM007618NINDS NIH HHS R01 NS033997NINDS NIH HHS R01 NS084057NINDS NIH HHS R35 NS097299
6 · The paper itself

Abstract

The N-methyl-d-aspartate-type glutamate receptor (NMDAR)-associated multiprotein complexes are indispensable for synaptic plasticity and cognitive functions. While purification and proteomic analyses of these signaling complexes have been performed in adult rodent and human brain, much less is known about the protein composition of NMDAR complexes in the developing brain and their modifications by neonatal hypoxic-ischemic (HI) brain injury. In this study, the postsynaptic density proteins were prepared from postnatal day 9 naïve, sham-operated and HI-injured mouse cortex. The GluN2B-containing NMDAR complexes were purified by immunoprecipitation with a mouse GluN2B antibody and subjected to mass spectrometry analysis for determination of the GluN2B binding partners. A total of 71 proteins of different functional categories were identified from the naïve animals as native GluN2B-interacting partners in the developing mouse brain. Neonatal HI reshaped the postsynaptic GluN2B interactome by recruiting new proteins, including multiple kinases, into the complexes; and modifying the existing associations within 1h of reperfusion. The early responses of postsynaptic NMDAR complexes and their related signaling networks may contribute to molecular processes leading to cell survival or death, brain damage and/or neurological disorders in term infants with neonatal encephalopathy.

Indexed as

AnimalsAnimals, NewbornBrainBrain ChemistryFemaleHypoxia-Ischemia, BrainImmunoprecipitationMaleMiceMice, Inbred C57BLNeural PathwaysNeuronsPregnancyPrimary Cell CultureProtein KinasesProteomicsNR2B NMDA receptorProtein KinasesReceptors, NeurotransmitterReceptors, N-Methyl-D-AspartateBrainDevelopmentHypoxia/ischemiaNMDA receptorsProteomics

Identifiers

PMID28993251
PMCPMC5723550
OpenAlexW2762011629

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.