ArticlePLoS neglected tropical diseases2017
Application of a loop-mediated isothermal amplification (LAMP) assay targeting cox1 gene for the detection of Clonorchis sinensis in human fecal samples.
Article in PLoS neglected tropical diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- The Development and Evaluation of a Loop-Mediated Isothermal Amplification (LAMP) Method for the Detection ofVeterinary sciences · 2026Article
- High sensitivity detection ofFood science and biotechnology · 2025Article
- Multiparasitism in Spain in a Korean Visiting Friends and Relatives: Case Report and Review of Imported Cases ofClinical case reports · 2025Article
- A point-of-care testing assay for clonorchiasis using a EuNPs-CsTR1 fluorescent probe-based immunoassay.PLoS neglected tropical diseases · 2024Article
- Clonorchiasis and opisthorchiasis: epidemiology, transmission, clinical features, morbidity, diagnosis, treatment, and control.Clinical microbiology reviews · 2024Review
- A rapid and visual detection assay for Clonorchis sinensis based on recombinase polymerase amplification and lateral flow dipstick.Parasites & vectors · 2023Article
- Optimization of loop-mediated isothermal amplification-based method for detection of macrolide-lincosamide-streptogramin B resistance inThe Indian journal of medical research · 2023Article
- Parallel detection of multiple zoonotic parasites using a real-time fluorogenic loop-mediated isothermal amplification-based quadruple-sample microfluidic chip.Frontiers in microbiology · 2023Article
- Detection ofPathogens (Basel, Switzerland) · 2022Article
- Point-of-care system for rapid real-time detection of SARS-CoV-2 virus based on commercially available Arduino platforms.Frontiers in bioengineering and biotechnology · 2022Article
- Elimination of Carryover Contamination in Real-Time Reverse Transcriptase Loop-Mediated Isothermal Amplification for Rapid Detection of the SARS-CoV-2 Virus in Point-of-Care Testing.Frontiers in cellular and infection microbiology · 2022Article
- LAMP Assay for the Detection ofPathogens (Basel, Switzerland) · 2021Article
- LAMP in Neglected Tropical Diseases: A Focus on Parasites.Diagnostics (Basel, Switzerland) · 2021Review
- Utility of an Automatic Vision-Based Examination System (AVE-562) for the Detection ofAnnals of laboratory medicine · 2021Article
- Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis for Neglected Parasitic Infections.International journal of molecular sciences · 2020Review
- Bile Ductal Transcriptome Identifies Key Pathways and Hub Genes in Clonorchis sinensis-Infected Sprague-Dawley Rats.The Korean journal of parasitology · 2020Article
- Rapid detection of Galba truncatula in water sources on pasture-land using loop-mediated isothermal amplification for control of trematode infections.Parasites & vectors · 2020Article
- Recent advances in nucleic acid-based methods for detection of helminth infections and the perspective of biosensors for future development.Parasitology · 2020Review
- Detection of helminths by loop-mediated isothermal amplification assay: a review of updated technology and future outlook.Infectious diseases of poverty · 2019Review
- A Simple and Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of SARS-CoV.Biochip journal · 2019Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundClonorchiasis is prevalent in the Far East, and a major health problem in endemic areas. Infected persons may experience, if not treated, serious complications such as bile stone formation, pyogenic cholangitis, and even cholangiocarcinoma. Early diagnosis and treatment are important to prevent serious complications and, therefore, the simple and reliable diagnostic method is necessary to control clonorchiasis in endemic areas, where resources for the diagnosis are limited. METHODOLOGY/PRINCIPLE
findingsThe loop-mediated isothermal amplification (LAMP) assay has been applied for the detection of Clonorchis sinensis DNA. Six primers targeting eight locations on the cytochrome c oxidase subunit 1 gene of C. sinensis were designed for species-specific amplification using the LAMP assay. The LAMP assay was sensitive enough to detect as little as 100 fg of C. sinensis genomic DNA and the detection limit in 100 mg of stool was as low as one egg. The assay was highly specific because no cross-reactivity was observed with the DNA of other helminths, protozoa or Escherichia coli. Then, LAMP assay was applied to human fecal samples collected from an endemic area of clonorchiasis in Korea. Using samples showing consistent results by both Kato-Katz method and real-time PCR as reference standards, the LAMP assay showed 97.1% (95% CI, 90.1-99.2) of sensitivity and 100% (95% CI, 92.9-100) of specificity. In stool samples with more than 100 eggs per gram of feces, the sensitivity achieved 100%.
conclusionsTo detect C. sinensis in human fecal samples, the LAMP assay was applied and achieved high sensitivity and specificity. The LAMP assay can be utilized in field laboratories as a powerful tool for diagnosis and epidemiological survey of clonorchiasis.
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