ArticleJournal of assisted reproduction and genetics2018
Sperm preparation after freezing improves motile sperm count, motility, and viability in frozen-thawed sperm compared with sperm preparation before freezing-thawing process.
Article in Journal of assisted reproduction and genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Article
- Protective effects of coenzyme Q10-supplemented cryopreservation medium on oxidative stress in frozen canine sperm.Veterinary research communications · 2026Article
- The Oxidative Stress of Human Sperm Cryopreservation.Antioxidants (Basel, Switzerland) · 2025Review
- The Influence of Cryopreservation on Sperm Morphology and Its Implications in Terms of Fractions of Higher-Quality Sperm.Journal of clinical medicine · 2024Article
- Seminal cell-free DNA as a potential marker for in vitro fertility of Nellore bulls.Journal of assisted reproduction and genetics · 2024Article
- Timing of semen cryopreservation: before or after processing?Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia · 2024Article
- Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects.International journal of molecular sciences · 2023Review
- Preservation of male fertility in patients undergoing pelvic irradiation.Reports of practical oncology and radiotherapy : journal of Greatpoland Cancer Center in Poznan and Polish Society of Radiation Oncology · 2023Review
- Beneficial effects of hypotaurine supplementation in preparation and freezing media on human sperm cryo-capacitation and DNA quality.Basic and clinical andrology · 2021Article
- Is pre-freeze sperm preparation more advantageous than post-freeze?Reproduction & fertility · 2021Article
- Combined use of medically-assisted reproductive techniques: a new bioethical issue.Acta bio-medica : Atenei Parmensis · 2019Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe aim of this study is to evaluate which cryopreservation protocol, freezing before or after swim-up, optimizes cryopreservation outcomes in terms of motile sperm count, motility, morphology, and viability, and also to establish whether sperm viability could be assessed based on sperm motility.
methodsFifty-three fresh and 53 swim-up prepared samples were considered for the first experiment. In parallel, total motility evaluation by CASA system (computer-assisted sperm analyzer) and hypoosmotic swelling test (HOS-test) was performed in each sample to compare the viability results of both methods. In the second experiment, 21 normozoospermic semen samples and 20 semen samples from male factor patients were included. After fresh ejaculate evaluation, the semen sample of each patient was divided into two aliquots, one of them was frozen before swim-up and the other was frozen after swim-up. Motility, sperm count, morphology, and viability were evaluated after thawing.
resultsA linear regression model allows prediction of HOS-test viability results based on total motility: HOS = 1.38 + 0.97 · TM (R
conclusionsTo conclude, total motility evaluation can predict HOS-test viability results, resulting in a more objective and less time-consuming method to assess viability. In addition, sperm freezing prior to swim-up selection must be considered in order to achieve better outcomes after thawing, especially in patients presenting poor sperm baseline.
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