ArticleMolecular medicine reports2017
miR‑494 inhibits cell proliferation and metastasis via targeting of CDK6 in osteosarcoma.
Article in Molecular medicine reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- MicroRNA signatures in osteosarcoma: diagnostic insights and therapeutic prospects.Molecular and cellular biochemistry · 2025Review
- The Landscape of microRNAs in Bone Tumor: A Comprehensive Review in Recent Studies.MicroRNA (Shariqah, United Arab Emirates) · 2024Review
- JAK1 Is a Novel Target of Tumor- and Invasion-Suppressive microRNA 494-5p in Colorectal Cancer.Cancers · 2023Article
- [Characteristics and microRNA expression profile of exosomes derived from odontogenic dental pulp stem cells].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2023Article
- Identification of Candidate MicroRNA-mRNA Subnetwork for Predicting the Osteosarcoma Progression by Bioinformatics Analysis.Computational and mathematical methods in medicine · 2022Article
- Circ_0081001 down-regulates miR-494-3p to enhance BACH1 expression and promotes osteosarcoma progression.Aging · 2021Article
- Circular RNA circ_0081001 knockdown enhances methotrexate sensitivity in osteosarcoma cells by regulating miR-494-3p/TGM2 axis.Journal of orthopaedic surgery and research · 2021Article
- Osteosarcoma and Metastasis.Frontiers in oncology · 2021Review
- MicroRNA-494 represses osteosarcoma development by modulating ASK-1 related apoptosis complexes.Translational cancer research · 2020Article
- Downregulated Long Non-Coding RNA MSC-AS1 Inhibits Osteosarcoma Progression and Increases Sensitivity to Cisplatin by Binding to MicroRNA-142.Medical science monitor : international medical journal of experimental and clinical research · 2020Article
- Circular RNA circ_ASAP2 promotes cell viability, migration, and invasion of gastric cancer cells by regulating the miR-770-5p/CDK6 axis.International journal of clinical and experimental pathology · 2020Article
- Integrative Clustering Reveals a Novel Subtype of Soft Tissue Sarcoma With Poor Prognosis.Frontiers in genetics · 2020Article
- miRNA signatures in childhood sarcomas and their clinical implications.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2019Review
- Current Status of Functional Studies on Circular RNAs in Bladder Cancer and their Potential Role as Diagnostic and Prognostic Biomarkers: A Review.Medical science monitor : international medical journal of experimental and clinical research · 2019Review
- miR-363-3p Inhibits Osteosarcoma Cell Proliferation and Invasion via Targeting SOX4.Oncology research · 2019Article
- MicroRNAs and their role in environmental chemical carcinogenesis.Environmental geochemistry and health · 2019Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tumorigenesis is a multistep process involving various cell growth‑associated factors. Accumulated evidence indicates that the disordered regulation of microRNAs (miRNAs) contributes to tumorigenesis. However, the detailed mechanism underlying the involvement of miRNAs in oncogenesis remains to be fully elucidated. In the present study, the repressed expression of microRNA (miR)‑494 was identified in 18 patients with osteosarcoma (OS) and OS cell lines, compared with corresponding controls. To determine whether deregulated miR‑494 exerts tumor‑suppressive effects in the development of OS, the effects of miR‑494 on cell proliferation and metastasis were evaluated. It was found that the restoration of miR‑494 in MG‑63 and U2OS cells led to inhibited cell proliferation and attenuated migratory propensity in vitro, determined through analysis using MTT, colony formation and Transwell assays. In addition, overexpression of miR‑494 markedly suppressed the tumor volume and weight in vivo. In accordance, the ectopic expression of miR‑494 induced cell cycle arrest at the G1/S phase in OS cells. Bioinformatics analysis and luciferase reporter assays were performed to investigate the potential regulatory role of miR‑494, the results of which indicated that miR‑494 directly targeted cyclin‑dependent kinase 6 (CDK6). Of note, the data obtained through reverse transcription‑quantitative polymerase chain reaction and western blot analyses suggested that the elevated expression of miR‑494 resulted in reduced mRNA and protein expression levels of CDK6. Taken together, these findings indicated that the miR‑494/CDK6 axis has a significant tumor‑suppressive effect on OS, and maybe a diagnostic and therapeutic target for the treatment of OS.
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Registered trials
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