ArticleIUCrJ2017
Single-particle cryo-EM using alignment by classification (ABC): the structure of
Article in IUCrJ, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Rescaling FSC curves.Acta crystallographica. Section D, Structural biology · 2025Article
- Alternatives for Uranyl Acetate Negative Staining With Respect to the Resolution Obtained After Single Particle Analysis of Erythrocruorin.Microscopy research and technique · 2025Article
- Elemental mapping in single-particle reconstructions by reconstructed electron energy-loss analysis.Nature methods · 2024Article
- Data-driven determination of number of discrete conformations in single-particle cryo-EM.Computer methods and programs in biomedicine · 2022Article
- Cryo-EM structure of the mature and infective Mayaro virus at 4.4 Å resolution reveals features of arthritogenic alphaviruses.Nature communications · 2021Article
- Improvement of cryo-EM maps by density modification.Nature methods · 2020Article
- Cryo-EM structures from sub-nl volumes using pin-printing and jet vitrification.Nature communications · 2020Article
- Myriapod haemocyanin: the first three-dimensional reconstruction ofOpen biology · 2020Article
- Validation tests for cryo-EM maps using an independent particle set.Journal of structural biology: X · 2020Article
- Cryo_fit: Democratization of flexible fitting for cryo-EM.Journal of structural biology · 2019Article
- Structure Determination by Single-Particle Cryo-Electron Microscopy: Only the Sky (and Intrinsic Disorder) is the Limit.International journal of molecular sciences · 2019Review
- Accurate flexible refinement of atomic models against medium-resolution cryo-EM maps using damped dynamics.BMC structural biology · 2018Article
- New tools for the analysis and validation of cryo-EM maps and atomic models.Acta crystallographica. Section D, Structural biology · 2018Article
- Mechanism for remodelling of the cell cycle checkpoint protein MAD2 by the ATPase TRIP13.Nature · 2018Article
Corrections and comments
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Authors and funding
10 authors.
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Abstract
Single-particle cryogenic electron microscopy (cryo-EM) can now yield near-atomic resolution structures of biological complexes. However, the reference-based alignment algorithms commonly used in cryo-EM suffer from reference bias, limiting their applicability (also known as the 'Einstein from random noise' problem). Low-dose cryo-EM therefore requires robust and objective approaches to reveal the structural information contained in the extremely noisy data, especially when dealing with small structures. A reference-free pipeline is presented for obtaining near-atomic resolution three-dimensional reconstructions from heterogeneous ('four-dimensional') cryo-EM data sets. The methodologies integrated in this pipeline include
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.