ArticleIUCrJ2017
Experimental phase determination with selenomethionine or mercury-derivatization in serial femtosecond crystallography.
Article in IUCrJ, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- A simple and practical approach for protein serial crystallography using grease matrix and large-area support film.Journal of synchrotron radiation · 2026Article
- Crystal structure of CmABCB1 multi-drug exporter in lipidic mesophase revealed by LCP-SFX.IUCrJ · 2022Article
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- High-throughput in situ experimental phasing.Acta crystallographica. Section D, Structural biology · 2020Article
- Viscosity-adjustable grease matrices for serial nanocrystallography.Scientific reports · 2020Article
- The complementarity of serial femtosecond crystallography and MicroED for structure determination from microcrystals.Current opinion in structural biology · 2019Review
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- Serial femtosecond crystallography at the SACLA: breakthrough to dynamic structural biology.Biophysical reviews · 2018Review
- Two-colour serial femtosecond crystallography dataset from gadoteridol-derivatized lysozyme for MAD phasing.Scientific data · 2017Article
- Article
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29 authors.
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Abstract
Serial femtosecond crystallography (SFX) using X-ray free-electron lasers (XFELs) holds enormous potential for the structure determination of proteins for which it is difficult to produce large and high-quality crystals. SFX has been applied to various systems, but rarely to proteins that have previously unknown structures. Consequently, the majority of previously obtained SFX structures have been solved by the molecular replacement method. To facilitate protein structure determination by SFX, it is essential to establish phasing methods that work efficiently for SFX. Here, selenomethionine derivatization and mercury soaking have been investigated for SFX experiments using the high-energy XFEL at the SPring-8 Angstrom Compact Free-Electron Laser (SACLA), Hyogo, Japan. Three successful cases are reported of single-wavelength anomalous diffraction (SAD) phasing using X-rays of less than 1 Å wavelength with reasonable numbers of diffraction patterns (13 000, 60 000 and 11 000). It is demonstrated that the combination of high-energy X-rays from an XFEL and commonly used heavy-atom incorporation techniques will enable routine
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