Evidence map›Paper›PMID 28989650›Full record

ArticleChemical science2017

Thiol-ene click chemistry: a biocompatible way for orthogonal bioconjugation of colloidal nanoparticles.

Yuan Liu, Weijia Hou, Hao Sun, Cheng Cui, Liqin Zhang, Ying Jiang, Yongxiang Wu, Yanyue Wang, Juan Li, Brent S Sumerlin and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed.

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  16. RSC advances · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yuan LiuMolecular Science and Biomedicine Laboratory , State Key Laboratory of Chemo/Bio-Sensing and Chemometrics , College of Life Sciences , College of Chemistry and Chemical Engineering , Aptamer Engineering Center of Hunan Province , Hunan University , Changsha , Hunan 410082 , China . Email: qlliu@iccas.ac.cn.
Weijia HouCenter for Research at Bio/Nano Interface , Department of Chemistry , Department of Physiology and Functional Genomics , Health Cancer Center , UF Genetics Institute , McKnight Brain Institute , University of Florida , Gainesville , Florida 32611-7200 , USA . Email: tan@chem.ufl.edu.
Hao SunGeorge & Josephine Butler Polymer Research Laboratory , Center for Macromolecular Science & Engineering , Department of Chemistry , University of Florida , Gainesville , Florida 32611-7200 , USA . Email: sumerlin@chem.ufl.edu.ORCID 0000-0001-9153-4021
Cheng CuiCenter for Research at Bio/Nano Interface , Department of Chemistry , Department of Physiology and Functional Genomics , Health Cancer Center , UF Genetics Institute , McKnight Brain Institute , University of Florida , Gainesville , Florida 32611-7200 , USA . Email: tan@chem.ufl.edu.
Liqin ZhangMolecular Science and Biomedicine Laboratory , State Key Laboratory of Chemo/Bio-Sensing and Chemometrics , College of Life Sciences , College of Chemistry and Chemical Engineering , Aptamer Engineering Center of Hunan Province , Hunan University , Changsha , Hunan 410082 , China . Email: qlliu@iccas.ac.cn.ORCID 0000-0002-3632-4117
Ying JiangMolecular Science and Biomedicine Laboratory , State Key Laboratory of Chemo/Bio-Sensing and Chemometrics , College of Life Sciences , College of Chemistry and Chemical Engineering , Aptamer Engineering Center of Hunan Province , Hunan University , Changsha , Hunan 410082 , China . Email: qlliu@iccas.ac.cn.
Yongxiang WuMolecular Science and Biomedicine Laboratory , State Key Laboratory of Chemo/Bio-Sensing and Chemometrics , College of Life Sciences , College of Chemistry and Chemical Engineering , Aptamer Engineering Center of Hunan Province , Hunan University , Changsha , Hunan 410082 , China . Email: qlliu@iccas.ac.cn.
Yanyue WangCenter for Research at Bio/Nano Interface , Department of Chemistry , Department of Physiology and Functional Genomics , Health Cancer Center , UF Genetics Institute , McKnight Brain Institute , University of Florida , Gainesville , Florida 32611-7200 , USA . Email: tan@chem.ufl.edu.
Juan LiMolecular Science and Biomedicine Laboratory , State Key Laboratory of Chemo/Bio-Sensing and Chemometrics , College of Life Sciences , College of Chemistry and Chemical Engineering , Aptamer Engineering Center of Hunan Province , Hunan University , Changsha , Hunan 410082 , China . Email: qlliu@iccas.ac.cn.
Brent S SumerlinGeorge & Josephine Butler Polymer Research Laboratory , Center for Macromolecular Science & Engineering , Department of Chemistry , University of Florida , Gainesville , Florida 32611-7200 , USA . Email: sumerlin@chem.ufl.edu.ORCID 0000-0001-5749-5444
Qiaoling LiuMolecular Science and Biomedicine Laboratory , State Key Laboratory of Chemo/Bio-Sensing and Chemometrics , College of Life Sciences , College of Chemistry and Chemical Engineering , Aptamer Engineering Center of Hunan Province , Hunan University , Changsha , Hunan 410082 , China . Email: qlliu@iccas.ac.cn.
Weihong TanMolecular Science and Biomedicine Laboratory , State Key Laboratory of Chemo/Bio-Sensing and Chemometrics , College of Life Sciences , College of Chemistry and Chemical Engineering , Aptamer Engineering Center of Hunan Province , Hunan University , Changsha , Hunan 410082 , China . Email: qlliu@iccas.ac.cn.ORCID 0000-0002-8066-1524

Funding

Development of molecular probes for biomedical applicationsR01GM079359 · NIGMS · UNIVERSITY OF FLORIDA · PI TAN, WEIHONG · 2007 to 2016
$2.3M
Molecular Target for Liver Cancer Diagnosis and TherapyR01CA133086 · NCI · UNIVERSITY OF FLORIDA · PI LIU, CHEN · 2009 to 2013
$1.5M
Expanded DNA, In Vitro Selection, Aptamers, and CancerR01GM111386 · NIGMS · FOUNDATION FOR APPLIED MOLECULAR EVOLUTN · PI BENNER, STEVEN A · 2014 to 2016
$1.2M
NCI NIH HHS R01 CA133086NIGMS NIH HHS R01 GM079359NIGMS NIH HHS R01 GM111386
6 · The paper itself

Abstract

Bioconjugation based on crosslinking primary amines to carboxylic acid groups has found broad applications in protein modification, drug development, and nanomaterial functionalization. However, proteins, which are made up of amino acids, typically give nonselective bioconjugation when using primary amine-based crosslinking. In order to control protein orientation and activity after conjugation, selective bioconjugation is desirable. We herein report an efficient and cysteine-selective thiol-ene click reaction-based bioconjugation strategy using colloidal nanoparticles. The resulting thiol-ene based aptamer and enzyme nanoconjugates demonstrated excellent target binding ability and enzymatic activity, respectively. Thus, thiol-ene click chemistry can provide a stable and robust crosslinker in a biocompatible manner for bioconjugation of any thiol-containing biomolecule with nanomaterials. This will open more opportunities for applications of thiol-ene reactions and functional colloidal nanoparticles in chemical biology.

Identifiers

PMID28989650
PMCPMC5628335

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.