ArticleParasitology research2017
Diversity of free-living amoebae in soils and their associated human opportunistic bacteria.
Article in Parasitology research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Draft genome sequence ofMicrobiology resource announcements · 2025Article
- Molecular Species Identification and Genotyping of Free-Living Amoebae in Soil of Recreational Mountain Areas in the Babiogórski National Park and Surroundings, Southern Poland.International journal of molecular sciences · 2025Article
- Impact of fluoroquinolone and heavy metal pollution on antibiotic resistance maintenance in aquatic ecosystems.Environmental microbiome · 2025Article
- Phosphonium chloride-based deep eutectic solvents inhibit pathogenic Acanthamoeba castellanii belonging to the T4 genotype.Folia microbiologica · 2025Article
- Extensive environmental survey of free-living amoebae and their elusive association with Mycobacterium bovis or Mycobacterium avium subsp. paratuberculosis.FEMS microbiology ecology · 2025Article
- Potentially Pathogenic Free-Living Amoebae Isolated from Soil Samples from Warsaw Parks and Squares.Pathogens (Basel, Switzerland) · 2024Article
- Phylogenomic, structural, and cell biological analyses reveal thatMicrobiology spectrum · 2024Article
- β-1,3-Glucan recognition byApplied and environmental microbiology · 2024Article
- Biochemical and structural characterization of an inositol pyrophosphate kinase from a giant virus.The EMBO journal · 2024Article
- Enhancement of capsular hypermucoviscosity in Klebsiella pneumoniae by Acanthamoeba.PLoS neglected tropical diseases · 2023Article
- Versatility ofHeliyon · 2023Review
- Recognition of Cell Wall Mannosylated Components as a Conserved Feature for Fungal Entrance, Adaptation and Survival Within Trophozoites ofFrontiers in cellular and infection microbiology · 2022Article
- Stimulation ofFrontiers in cell and developmental biology · 2022Article
- Article
- Commensals Serve as Natural Barriers to Mammalian Cells during Acanthamoeba castellanii Invasion.Microbiology spectrum · 2021Article
- Environmental Free-Living Amoebae Can Predate on Diverse Antibiotic-Resistant Human Pathogens.Applied and environmental microbiology · 2021Article
- Advances in the Microbiology of Stenotrophomonas maltophilia.Clinical microbiology reviews · 2021Article
- Intracellular Microbiome Profiling of thePathogens (Basel, Switzerland) · 2021Article
- Microbiological and Chemical Assessment of Wastewater Discharged by Infiltration Trenches in Fractured and Karstified Limestone (SCA.Re.S. Project 2019-2020).Pathogens (Basel, Switzerland) · 2020Article
- Biosynthesis of Polyhydroxyalkanoates (PHAs) by the Valorization of Biomass and Synthetic Waste.Molecules (Basel, Switzerland) · 2020Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Free-living amoebae (FLA) are ubiquitous protozoa found worldwide in the environment. They feed by phagocytosis on various microorganisms. However, some bacteria, i.e., amoebae-resistant bacteria (ARB) or bacterial endocytobionts, can resist phagocytosis and even multiply inside FLA. This study investigated the diversity of culturable FLA in various soils from agricultural and mining sites and their bacterial endocytobionts. FLA were cultured on non-nutrient agar with alive Escherichia coli and identified by PCR and sequencing. Amoebae were lysed and bacterial endocytobionts were cultured on TSA 1/10 and Drigalski medium. Bacterial isolates were identified by PCR and 16S rDNA sequencing and characterized for their antibiotic resistance properties. To measure bacterial virulence, the amoebal model Dictyostelium discoideum was used. The analysis of FLA diversity showed that Tetramitus was the most prevalent genus in agricultural soil from Burkina Faso (73%) and garden soil from Vietnam (42%) while Naegleria and Acanthamoeba were dominant genera in mining soil from Vietnam (55%) and French alpine soil (77%). Some genera were only present in one out of the four soils analyzed. The bacterial endocytobiont included Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria. Human opportunistic pathogens identified as Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Burkholderia cepacia were found associated with amoebae including Micriamoeba, Tetramitus, Willaertia, or Acanthamoeba. Some of these bacteria showed various antibiotic resistance phenotypes and were virulent. Our study confirms that the occurrence of these opportunistic bacteria with FLA in soils may be important for the survival, multiplication, and spread of pathogens in the environment.
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