ArticleExtremophiles : life under extreme conditions2017
Production of the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with varied composition using different nitrogen sources with Haloferax mediterranei.
Article in Extremophiles : life under extreme conditions, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- A kinetic model for polyhydroxyalkanoates production by Cupriavidus necator using dark fermentation effluents.Biodegradation · 2026Article
- Advanced Strategies for Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production: PHA Synthase Homologous Overexpression in the ExtremophileMarine drugs · 2025Article
- Bioplastic Production from Agri-Food Waste through the Use ofMicroorganisms · 2024Review
- ALACEN: A Holistic Herbaceous Biomass Fractionation Process Attaining a Xylose-Rich Stream for Direct Microbial Conversion to Bioplastics.ACS sustainable chemistry & engineering · 2024Article
- Production of Polyhydroxyalkanoates for Biodegradable Food Packaging Applications UsingFoods (Basel, Switzerland) · 2024Review
- Production of polyhydroxybutyrate by coupled saccharification-fermentation of inulin.Bioprocess and biosystems engineering · 2024Article
- Process Development of Polyhydroxyalkanoates Production by Halophiles Valorising Food Waste.Bioengineering (Basel, Switzerland) · 2022Article
- Extremophilic BacteriumFrontiers in bioengineering and biotechnology · 2022Article
- Exploitation of wasted bread as substrate for polyhydroxyalkanoates production through the use ofFrontiers in microbiology · 2022Article
- Evaluating haloarchaeal culture media for ultrahigh-molecular-weight polyhydroxyalkanoate biosynthesis by Haloferax mediterranei.Applied microbiology and biotechnology · 2021Article
- Optimization of nitrogen source supply for enhanced biosynthesis and quality of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by extremely halophilic archaeon Haloferax mediterranei.MicrobiologyOpen · 2020Article
- Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory.Microbial cell factories · 2020Review
- Polyhydroxyalkanoate Biosynthesis at the Edge of Water Activitiy-Haloarchaea as Biopolyester Factories.Bioengineering (Basel, Switzerland) · 2019Review
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Abstract
The extreme haloarchaea Haloferax mediterranei accumulates poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) without the need for specific precursors. In this study, growth kinetics and PHBV synthesis were characterised under nitrogen-excess and nitrogen-limiting conditions in ammonium and, for the first time, nitrate. With excess nitrogen, ammonium and nitrate cultures generated 10.7 g/L biomass containing 4.6 wt% PHBV and 5.6 g/L biomass with 9.3 wt% PHBV, respectively. Copolymer composition varied with the nitrogen source used: PHBV from ammonium cultures had 16.9 mol% 3-hydroxyvalerate (HV), while PHBV from nitrate cultures contained 12.5 mol% HV. Nitrogen limitation was achieved with carbon-to-nitrogen (C/N) molar ratios of 25 or higher. Nitrogen limitation reduced biomass generation and polymer concentration, but polymer accumulation increased to 6.6 and 9.4% for ammonium and nitrate, respectively, with C/N 42. PHBV composition was also affected and cultures with lower C/N ratios produced richer HV polymers. Copolymer formation was not a uniform process: HV was only detected after a minimum accumulation of 0.45 g/L PHB and lasted for a maximum of 48 h. The understanding of copolymer synthesis and the influence of culture conditions such as the nitrogen source will help in designing novel strategies for the production of PHBV with more regular structure and material properties.
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