Evidence map›Paper›PMID 28988312›Full record

ArticleKnee surgery, sports traumatology, arthroscopy : official journal of the ESSKA2018

Contamination occurs during ACL graft harvesting and manipulation, but it can be easily eradicated.

Daniel Pérez-Prieto, María E Portillo, Raúl Torres-Claramunt, Xavier Pelfort, Pedro Hinarejos, Joan C Monllau

Abstract read
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In one paragraph

Article in Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed, 2 pooled it
5.0field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 2 syntheses or guidelines pooled it, 86 citations in OpenAlex.

  1. Pooled it
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  13. In Vitro Study of Gentamicin Elution from Tendon Grafts.Indian journal of orthopaedics · 2023
    Article
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  17. Allograft contamination during suture preparation for anterior cruciate ligament reconstruction: an ex vivo study.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Daniel Pérez-PrietoDepartment of Orthopaedic Surgery and Traumatology, Hospital del Mar, Universitat Autònoma de Barcelona (UAB), Passeig Marítim, 25, Barcelona, Spain. dperezprieto@parcdesalutmar.cat.
María E PortilloMicrobiology Department, Hospital de Navarra, Pamplona, Spain.
Raúl Torres-ClaramuntDepartment of Orthopaedic Surgery and Traumatology, Hospital del Mar, Universitat Autònoma de Barcelona (UAB), Passeig Marítim, 25, Barcelona, Spain.
Xavier PelfortOrthopedic Surgery, Consorci Sanitari de l'Anoia, Igualada, Spain.
Pedro HinarejosDepartment of Orthopaedic Surgery and Traumatology, Hospital del Mar, Universitat Autònoma de Barcelona (UAB), Passeig Marítim, 25, Barcelona, Spain.
Joan C MonllauDepartment of Orthopaedic Surgery and Traumatology, Hospital del Mar, Universitat Autònoma de Barcelona (UAB), Passeig Marítim, 25, Barcelona, Spain.
Universitat Autònoma de Barcelona · ESHospital d'Igualada · ESUniversidad de Navarra · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeWhy anterior cruciate ligament (ACL) autograft soaking in a 5 mg/ml vancomycin solution decreases the rate of infection has not been well-explained. One hypothesis is that grafts can be contaminated during harvesting and vancomycin eradicates the bacteria. The purpose of the present study is to assess how the vancomycin solution acts against ACL graft contamination during graft harvesting and preparation.

methodsThe study was carried out in three university hospitals over a period of 6 months. After sample size calculation, 50 patients were included in the study. Three samples were taken from each ACL graft. Sample 1 was obtained immediately after graft harvesting. After graft manipulation and preparation, the remaining tissue was divided into two parts. The raw sample was denominated sample 2 and sample 3 consisted of the rest of the remaining tissue that had been soaked in the vancomycin solution. All the cultures were incubated at 37 °C with 5% CO

resultsIn seven cases (14%), either sample 1 or sample 2 was positive. In five of the cases (10%), only the sample after graft preparation was positive (sample 2). In two cases (4%), sample 1 and sample 2 were positive for the same bacteria. Isolated microorganisms corresponded to coagulase-negative staphylococci (CNS) and Propionibacterium acnes. No bacterial growth was observed in sample 3 (p < 0.001). Thus, none of those seven positive cases (0%) were positive after vancomycin soaking (p < 0.001).

conclusionIn the series, ACL graft harvesting and manipulation leads to bacterial contamination in 14% of the cases. This contamination is fully eradicated after soaking in the vancomycin solution in this series. LEVEL OF EVIDENCE: Level II.

Indexed as

AdolescentAdultAnterior Cruciate LigamentAnterior Cruciate Ligament InjuriesAnterior Cruciate Ligament ReconstructionAnti-Bacterial AgentsFemaleHumansMaleSurgical Wound InfectionTransplantation, AutologousTransplantsVancomycinAnti-Bacterial AgentsVancomycinACL contaminationACL infectionBiofilmInfection preventionVancomycin soaking

Identifiers

PMID28988312
OpenAlexW2762232059

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.