ArticleBiotechnology and bioengineering2018
Enzymatic synthesis of chiral amino-alcohols by coupling transketolase and transaminase-catalyzed reactions in a cascading continuous-flow microreactor system.
Article in Biotechnology and bioengineering, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Article
- Engineering ω-transaminase for efficient dihydroxyacetone transamination in serinol biosynthesis starting from methanol.Synthetic and systems biotechnology · 2026Article
- Integrating biocatalysis with continuous flow: current status, challenges, and future perspectives.Journal of advanced research · 2026Review
- Two-step tandem synthesis of sugar-containing pyrimidine derivatives catalyzed by Lipozyme® TL IM in continuous-flow microreactors.RSC advances · 2024Article
- Lipase-Catalyzed Strategies for the Preparation of Enantiomeric THIQ and THMolecules (Basel, Switzerland) · 2023Review
- Biocatalytic Transamination of Aldolase-Derived 3-Hydroxy Ketones.Advanced synthesis & catalysis · 2023Article
- Biocatalysis in Continuous-Flow Microfluidic Reactors.Advances in biochemical engineering/biotechnology · 2022Article
- High Regio- and Stereoselective Multi-enzymatic Synthesis of All Phenylpropanolamine Stereoisomers from β-Methylstyrene.Chembiochem : a European journal of chemical biology · 2021Article
- Parallel Interconnected Kinetic Asymmetric Transformation (PIKAT) with an Immobilized ω-Transaminase in Neat Organic Solvent.Molecules (Basel, Switzerland) · 2020Article
- Continuous Flow Bioamination of Ketones in Organic Solvents at Controlled Water Activity using Immobilized ω-Transaminases.Advanced synthesis & catalysis · 2020Article
- Hydrogel Microvalves as Control Elements for Parallelized Enzymatic Cascade Reactions in Microfluidics.Micromachines · 2020Article
- Aminopolyols from Carbohydrates: Amination of Sugars and Sugar-Derived Tetrahydrofurans with Transaminases.Angewandte Chemie (International ed. in English) · 2019Article
- Extending Designed Linear Biocatalytic Cascades for Organic Synthesis.ChemCatChem · 2019Review
- Continuous flow biocatalysis.Chemical Society reviews · 2018Review
- Enzymatic synthesis of chiral amino-alcohols by coupling transketolase and transaminase-catalyzed reactions in a cascading continuous-flow microreactor system.Biotechnology and bioengineering · 2018Article
- Bioprocess microfluidics: applying microfluidic devices for bioprocessing.Current opinion in chemical engineering · 2017Review
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12 authors.
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Abstract
Rapid biocatalytic process development and intensification continues to be challenging with currently available methods. Chiral amino-alcohols are of particular interest as they represent key industrial synthons for the production of complex molecules and optically pure pharmaceuticals. (2S,3R)-2-amino-1,3,4-butanetriol (ABT), a building block for the synthesis of protease inhibitors and detoxifying agents, can be synthesized from simple, non-chiral starting materials, by coupling a transketolase- and a transaminase-catalyzed reaction. However, until today, full conversion has not been shown and, typically, long reaction times are reported, making process modifications and improvement challenging. In this contribution, we present a novel microreactor-based approach based on free enzymes, and we report for the first time full conversion of ABT in a coupled enzyme cascade for both batch and continuous-flow systems. Using the compartmentalization of the reactions afforded by the microreactor cascade, we overcame inhibitory effects, increased the activity per unit volume, and optimized individual reaction conditions. The transketolase-catalyzed reaction was completed in under 10 min with a volumetric activity of 3.25 U ml
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