Evidence map›Paper›PMID 28986832›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Assays to Assess Arenaviral Glycoprotein Function.

Junjie Shao, Xiaoying Liu, Yuying Liang, Hinh Ly

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 84% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Junjie ShaoDepartment of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities, 1988 Fitch Ave., 295 AS/VM Bldg., Saint Paul, MN, 55108, USA.
Xiaoying LiuDepartment of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities, 1988 Fitch Ave., 295 AS/VM Bldg., Saint Paul, MN, 55108, USA.
Yuying LiangDepartment of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities, 1988 Fitch Ave., 295 AS/VM Bldg., Saint Paul, MN, 55108, USA.
Hinh LyDepartment of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities, 1988 Fitch Ave., 295 AS/VM Bldg., Saint Paul, MN, 55108, USA. hly@umn.edu.
University of Minnesota · US

Funding

Yessinia autotransporters (Yaps): Structure, function, and host responseU54AI057157 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI GOLDMAN, WILLIAM E · 2003 to 2013
$91.4M
Molecular determinants of virulent arenavirus infection in hostsR01AI083409 · NIAID · UNIVERSITY OF MINNESOTA · PI LIANG, YUYING · 2010 to 2014
$2.1M
Novel Mechanism of Immune Evasion by Arenaviral NucleoproteinsR01AI093580 · NIAID · UNIVERSITY OF MINNESOTA · PI LY, HINH · 2012 to 2016
$1.9M
NIAID NIH HHS R01 AI083409NIAID NIH HHS R01 AI093580NIAID NIH HHS U54 AI057157
6 · The paper itself

Abstract

Arenaviruses, such as Lassa virus (LASV) and Pichindé virus (PICV), are enveloped viruses with a bi-segmented ambisense RNA genome. The large (L) genomic segment encodes the Z matrix protein and the L RNA-dependent RNA polymerase, whereas the small (S) genomic segment encodes the nucleoprotein (NP) and the glycoprotein precursor complex (GPC). GPC is processed by signal peptidase in the endoplasmic reticulum into the stable signal peptide (SSP) and GP1/GP2, which is further cleaved by the Golgi-resident subtilisin kexin isozyme-1 (SKI-1)/site-1 protease (S1P) into the cellular receptor-recognition subunit GP1 and the transmembrane subunit GP2, which helps promote the membrane fusion reaction to allow virus entry into the cell. This article describes assays to assess PICV GPC expression, proteolytic processing, fusion function, and GPC-mediated virus-like particle (VLP) entry into cells under tissue-culture conditions.

Indexed as

AnimalsArenavirusBiological AssayGlycoproteinsHumansLassa virusPichinde virusProprotein ConvertasesSerine EndopeptidasesViral Envelope ProteinsGlycoproteinsmembrane-bound transcription factor peptidase, site 1Proprotein ConvertasesSerine EndopeptidasesViral Envelope ProteinsArenavirusCell entryCell membrane fusionGlycoprotein precursor complexGPCLassa virus (LASV)Pichindé virus (PICV)Receptor recognition

Identifiers

PMID28986832
PMCPMC6386583
OpenAlexW2763286018

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.