Evidence map›Paper›PMID 28986817›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Isobaric Labeling-Based LC-MS/MS Strategy for Comprehensive Profiling of Human Pancreatic Tissue Proteome.

Chih-Wei Liu, Qibin Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Quantitative Phosphoproteomic Analysis Reveals the Regulatory Networks ofInternational journal of molecular sciences · 2020
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Chih-Wei LiuCenter for Translational Biomedical Research, University of North Carolina at Greensboro, North Carolina Research Campus, 500 Laureate Way Suite 4226, Kannapolis, NC, 28081, USA.
Qibin ZhangCenter for Translational Biomedical Research, University of North Carolina at Greensboro, North Carolina Research Campus, 500 Laureate Way Suite 4226, Kannapolis, NC, 28081, USA. q_zhang2@uncg.edu.

Funding

Protein Markers to Islet Autoimmunity and T1D ProgressionR01DK114345 · NIDDK · UNIVERSITY OF NORTH CAROLINA GREENSBORO · PI ZHANG, QIBIN · 2017 to 2022
$1.7M
NIDDK NIH HHS R01 DK114345
6 · The paper itself

Abstract

The pancreas is an organ with both endocrine and exocrine functions, and various pathologies, such as pancreatic cancer and diabetes are associated with this organ. Owing to the limited pancreatic biopsy samples available for research, it is critical to make the best use of cadaveric pancreatic tissue for biomarker studies and mechanistic understanding of pancreas-related pathologies. Discovery-phase quantitative proteomics has attracted a lot of attention for its capabilities in large-scale protein identification and accurate protein quantification. Here, we describe a workflow using isobaric labeling (tandem mass tag or TMT) based quantitative proteomics to confidently identify and quantify human pancreatic tissue proteome, including sample preparation, isobaric tag labeling, peptide level fractionation, LC-MS/MS, database search, and statistical analysis.

Indexed as

Chromatography, Reverse-PhaseHumansPancreasPeptidesProteomeProteomicsStaining and LabelingTandem Mass SpectrometryPeptidesProteomeFractionationHuman pancreatic tissueIsobaric labelingLC-MS/MSMaxQuantPerseusTandem mass tagTissue proteome

Identifiers

PMID28986817
PMCPMC6320219

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.