Evidence map›Paper›PMID 28986672›Full record

ArticleTransgenic research2017

High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza.

Yanaysi Ceballo, Kenia Tiel, Alina López, Gleysin Cabrera, Marlene Pérez, Osmany Ramos, Yamilka Rosabal, Carlos Montero, Rima Menassa, Ann Depicker and 1 more

Abstract read
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In one paragraph

Article in Transgenic research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 3 countries.

Yanaysi CeballoPlant Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), PO Box 6162, 10600, Havana, Havana, Cuba. yanaysi.ceballo@cigb.edu.cu.
Kenia TielPlant Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), PO Box 6162, 10600, Havana, Havana, Cuba.
Alina LópezPlant Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), PO Box 6162, 10600, Havana, Havana, Cuba.
Gleysin CabreraDepartment of Carbohydrate Chemistry, Center for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
Marlene PérezPlant Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), PO Box 6162, 10600, Havana, Havana, Cuba.
Osmany RamosPlant Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), PO Box 6162, 10600, Havana, Havana, Cuba.
Yamilka RosabalPlant Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), PO Box 6162, 10600, Havana, Havana, Cuba.
Carlos MonteroAnimal Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), Havana, Cuba.
Rima MenassaLondon Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada.
Ann DepickerDepartment Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
Abel HernándezPlant Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), PO Box 6162, 10600, Havana, Havana, Cuba.
Centro de Ingeniería Genética y Biotecnología · CULondon Research and Development CentreVIB-UGent Center for Plant Systems Biology · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tobacco seeds can be used as a cost effective system for production of recombinant vaccines. Avian influenza is an important respiratory pathogen that causes a high degree of mortality and becomes a serious threat for the poultry industry. A safe vaccine against avian flu produced at low cost could help to prevent future outbreaks. We have genetically engineered tobacco plants to express extracellular domain of hemagglutinin protein from H5N1 avian influenza virus as an inexpensive alternative for production purposes. Two regulatory sequences of seed storage protein genes from Phaseolus vulgaris L. were used to direct the expression, yielding 3.0 mg of the viral antigen per g of seeds. The production and stability of seed-produced recombinant HA protein was characterized by different molecular techniques. The aqueous extract of tobacco seed proteins was used for subcutaneous immunization of chickens, which developed antibodies that inhibited the agglutination of erythrocytes after the second application of the antigen. The feasibility of using tobacco seeds as a vaccine carrier is discussed.

Indexed as

Agglutination TestsAnimalsChickensChromatography, High Pressure LiquidGene Expression Regulation, PlantHemagglutinin Glycoproteins, Influenza VirusInfluenza in BirdsInfluenza VaccinesNicotianaPhaseolusPlants, Genetically ModifiedPolysaccharidesRecombinant ProteinsSeedshemagglutinin, avian influenza A virusHemagglutinin Glycoproteins, Influenza VirusInfluenza VaccinesPolysaccharidesRecombinant ProteinsAvian influenzaHemagglutininPhaseolin promoterTobacco seed

Identifiers

PMID28986672
OpenAlexW2763362682

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.