Evidence map›Paper›PMID 28985565›Full record

ArticleCell2017

Genetic Mapping and Biochemical Basis of Yellow Feather Pigmentation in Budgerigars.

Thomas F Cooke, Curt R Fischer, Ping Wu, Ting-Xin Jiang, Kathleen T Xie, James Kuo, Elizabeth Doctorov, Ashley Zehnder, Chaitan Khosla, Cheng-Ming Chuong and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.

0numbers the graph read from it
0cells of the map it votes in
64citing papers in PubMed
27.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

64 citing papers in PubMed, 138 citations in OpenAlex.

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  7. Widespread variation inbioRxiv : the preprint server for biology · 2025
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4 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Thomas F CookeDepartment of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
Curt R FischerChEM-H, Stanford University, Stanford, CA 94305, USA; Stanford Genome Technology Center, Stanford University, Stanford, CA 94305, USA.
Ping WuDepartment of Pathology, University of Southern California, Los Angeles, CA 90033, USA.
Ting-Xin JiangDepartment of Pathology, University of Southern California, Los Angeles, CA 90033, USA.
Kathleen T XieDepartment of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA.
James KuoDepartment of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
Elizabeth DoctorovDepartment of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
Ashley ZehnderDepartment of Biomedical Data Science, Stanford University School of Medicine, Stanford, CA 94305, USA.
Chaitan KhoslaChEM-H, Stanford University, Stanford, CA 94305, USA; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA; Departments of Chemistry, Stanford University, Stanford, CA 94305, USA.
Cheng-Ming ChuongDepartment of Pathology, University of Southern California, Los Angeles, CA 90033, USA; Integrative Stem Cell Center, China Medical University, Taichung 404, Taiwan; Center for the Integrative and Evolutionary Galliformes Genomics, National Chung Hsing University, Taichung 402, Taiwan.
Carlos D BustamanteDepartment of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Biomedical Data Science, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: cdbustam@stanford.edu.
Stanford University · USUniversity of Southern California · USChina Medical University · TW

Funding

Supplement to Enhance Wellness and Resiliency in the Graduate EnvironmentT32GM007276 · NIGMS · STANFORD UNIVERSITY · PI MORRISON, ASHBY J. · 1985 to 2023
$32.9M
INSTITUTIONAL TRAINING GRANT IN GENOME SCIENCET32HG000044 · NHGRI · STANFORD UNIVERSITY · PI MICHAEL P. SNYDER · 1995 to 2026
$32.2M
Size regulation of skin appendagesR01AR047364 · NIAMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Cheng-Ming Chuong · 2002 to 2026
$10.1M
Tissue Engineering of New Hair FormationR01AR060306 · NIAMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI CHUONG, CHENG-MING · 2010 to 2019
$4.6M
Tissue Patterning in living skin and organ explantsR37AR060306 · NIAMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Cheng-Ming Chuong · 2020 to 2026
$3.6M
NHGRI NIH HHS T32 HG000044NIAMS NIH HHS R01 AR047364NIAMS NIH HHS R01 AR060306NIAMS NIH HHS R37 AR060306NIGMS NIH HHS T32 GM007276
6 · The paper itself

Abstract

Parrot feathers contain red, orange, and yellow polyene pigments called psittacofulvins. Budgerigars are parrots that have been extensively bred for plumage traits during the last century, but the underlying genes are unknown. Here we use genome-wide association mapping and gene-expression analysis to map the Mendelian blue locus, which abolishes yellow pigmentation in the budgerigar. We find that the blue trait maps to a single amino acid substitution (R644W) in an uncharacterized polyketide synthase (MuPKS). When we expressed MuPKS heterologously in yeast, yellow pigments accumulated. Mass spectrometry confirmed that these yellow pigments match those found in feathers. The R644W substitution abolished MuPKS activity. Furthermore, gene-expression data from feathers of different bird species suggest that parrots acquired their colors through regulatory changes that drive high expression of MuPKS in feather epithelia. Our data also help formulate biochemical models that may explain natural color variation in parrots. VIDEO ABSTRACT.

Indexed as

Amino Acid SequenceAnimalsAvian ProteinsFeathersGene ExpressionGenomeGenome-Wide Association StudyMelopsittacusPigmentationPigments, BiologicalPolyenesPolyketide SynthasesPolymorphism, Single NucleotideRegenerationSequence AlignmentAvian ProteinsPigments, BiologicalPolyenesPolyketide Synthasesartificial selectionbudgerigarfeather colorHi-CmendelianParrotpigmentationpolyenepolyketide synthasepsittacofulvinspecialized metabolismtrait mapping

Identifiers

PMID28985565
PMCPMC5951300
OpenAlexW2762269578

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.